CORE--ELECTRON MICROSCOPY

DERC电子显微镜(EM)核心设施的目标是为UMMS的DERC研究人员提供完整的、一流的EM服务。该设施位于基础和临床科学大楼地下室一个新装修的套房的中心位置。它由George Witman博士和Roger Craig博士指导，并由Gregory Hendricks博士进行日常管理，Gregory Hendricks博士是一名全职专业电子显微镜专家，在几乎所有EM方法方面都有丰富的经验。设备包括飞利浦CM120、CM12和CM10透射电子显微镜和一台ETEC AutoSCAN扫描电子显微镜、BalzzBAF400T冷冻蚀刻系统、三台Reichert Ultraut E冷冻超微显微镜和其他样品制备设备。技术包括免疫-EM、冷冻-EM和常规薄层法制备样品 切片、阴性染色、金属阴影、冷冻显微切割、快速冷冻、冷冻替代、冷冻断裂/冷冻蚀刻和临界点干燥。亨德里克斯博士在一名助手的帮助下，可以提供从标本准备到电子显微照片打印的所有服务。或者，调查人员可以使用设施试剂和设备来进行他们自己的标本准备和检查，并根据需要得到设施工作人员的协助。因此，对于没有新兴市场经验的用户和具有大量新兴市场经验的用户来说，该工具同样有效。通过将EM的所有方面集中在该设施中，DERC 以最具成本效益和时间效益的方式为其成员提供尽可能最好的EM服务。该机制向所有UMMS研究人员开放，由机构资金和用户按存储容量使用计费相结合提供支持；DERC调查人员收取的费率是非附属UMMS研究人员的一半。许多DERC成员将EM作为其研究的一个组成部分，DERC成员发表了大量的论文，报告了EM核心基金取得的成果。

The goal of the DERC Electron Microscopy (EM) Core Facility is to provide complete, first-rate EM service to DERC researchers at UMMS. The Facility is centrally located in a newly renovated suite in the basement of the Basic and Clinical Sciences Building. It is directed by Drs. George Witman and Roger Craig, and managed on a day-to-day basis by Dr. Gregory Hendricks, a full-time professional electron microscopist with extensive experience in virtually all EM methods. Equipment includes Philips CM120, CM12 and CM10 transmission electron microscopes and an ETEC Autoscan scanning electron microscope, a Balzers BAF400T freeze-etching system; three Reichert Ultracut E cryo-ultramicrotomes, and other equipment for specimen preparation. Techniques include immuno-EM, cryo-EM, and preparation of specimens by conventional thin sectioning, negative staining, metal shadowing, cryoultramicrotomy, rapid freezing, freeze substitution, freeze-fracture/freeze-etch, and critical point drying. Dr. Hendricks, aided by an assistant, can provide all services from specimen preparation to printing of electron micrographs. Alternatively, investigators may use Facility reagents and equipment to carry out their own specimen preparation and examination, with assistance from Facility staff as needed. Consequently, the Facility works just as well for users with no EM experience as for those with considerable EM experience. By centralizing all aspects of EM in this facility, the DERC provides its members with the best possible EM services in the most cost- and time-effective manner. The Facility, which is open to all UMMS researchers, is supported by a combination of institutional funds and user chargebacks; DERC investigators are charged at one-half the rate for non-affiliated UMMS researchers. Many DERC members use EM as an integral part of their research, and DERC members have published numerous papers that report results obtained in the EM Core Facility.