Engineering enhanced vitamin synthesis in bacteria

工程增强细菌中的维生素合成

• 批准号: 2750309
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2750309
• 关键词: Engineering; enhanced; vitamin; synthesis; bacteria

This project aims to apply synthetic biology and metabolic engineering strategies to one of the most complex biochemical pathways found in nature in order to address a current need for a cheaper and more reliable source of vitamin B12, the so-called anti-pernicious anaemia factor. Vitamin B12 is a cobalt-containing molecule that acts as a coenzyme and cofactor for a number of key biological processes. It is unique among the vitamins in that it is made solely by prokaryotes but is retained by many eukaryotes as a nutrient because of the greatly improved rates of reaction observed with B12-dependent enzymes in comparison to B12-independent processes. Vitamin B12 is a structurally complex molecule and this is reflected in an equally complex biochemical pathway. This collaborative project seeks to provide new key fundamental insights into the operations of the B12 biosynthetic pathway and at the same time lead to the construction of a strong B12-overproduction strain.

该项目旨在将合成生物学和代谢工程策略应用于自然界中发现的最复杂的生物化学途径之一，以解决目前对更便宜，更可靠的维生素B12来源的需求，即所谓的抗恶性贫血因子。维生素B12是一种含钴分子，作为辅酶和辅因子的一些关键的生物过程。它在维生素中的独特之处在于它仅由原核生物产生，但由于与B12独立过程相比，B12依赖性酶的反应速率大大提高，因此许多真核生物将其作为营养素保留。维生素B12是一种结构复杂的分子，这反映在一个同样复杂的生化途径。该合作项目旨在为B12生物合成途径的运作提供新的关键基本见解，同时导致构建强大的B12过量生产菌株。