ASSESSMENT OF THE INFLUENCE OF INTRAMUSCULAR LIPIDS ON THE PATHOLOGY OF INSULIN

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。本初步研究的目的是测试饮食加运动在没有减肥的情况下消耗肌内脂（IML）的能力，通过测量IML消耗前后的胰岛素敏感性来评估IML对全身胰岛素敏感性的影响，并确定IML消耗或胰岛素敏感性变化的任何种族差异。为了评估这一点，12名年龄在19岁到45岁之间的健康、久坐不动的男性（6名黑人和6名白人，年龄和体重指数配对）在一周内食用低脂（少于每日热量的10%）、中等纤维（每天25克左右）等热量饮食。受试者将在基线和控制饮食和运动7天后进行人体测量、DXA体成分分析、胰岛素敏感性测试和抽血（空腹血清胆固醇、HDL、LDL、甘油三酯、游离脂肪酸、瘦素和脂联素水平）。本研究的假设是：低脂饮食加运动会在没有减肥的情况下消耗IML； IML的消耗会对胰岛素敏感性产生独立于减肥的积极影响；IML的种族差异会导致胰岛素敏感性变化的差异。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this pilot study is to test the ability of diet plus exercise to deplete intramuscular lipids (IML) in the absence of weight-loss, to assess the influence of IML on whole-body insulin sensitivity by measuring insulin sensitivity before and after IML depletion, and to identify any racial differences in IML depletion or insulin sensitivity changes. To assess this, 12 healthy, sedentary men (6 Black and 6 White, pair-matched for age and body mass index) between the ages of 19 and 45 will consume a very low-fat (less than 10% of daily calories), moderate-fiber (>25 grams/day), isocaloric diet for one week. The subjects will undergo measurement of body anthropometics, body composition analysis using DXA, insulin sensitivity testing, and blood draws (for fasting serum cholesterol, HDL, LDL, triglyceride, free fatty acids, leptin, and adiponectin levels) at baseline and after 7 days of controlled diet and exercise. The hypotheses of this study are that a low-fat diet plus exercise will deplete IML in the absence of weight-loss, that depletion of IML will have a positive effect on insulin sensitivity that is independent of weight loss, and that there will be racial differences in IML resulting in differences in insulin sensitivity changes.