SKELETAL MUSCLE INJURY: ENERGY METAB, MUSCLE PERFUSION & EXERCISE PERFORMANCE

维持大脑的磷酸化状态， 细胞活力和正常的大脑功能，需要 葡萄糖和氧气的不受阻碍的供应 在氧气的条件下 不足，某些调节剂（如ATP，G-6-P，G-1， 6-DP）可能通过其对葡萄糖的作用提高葡萄糖的利用率。 各自代谢途径中的关键酶。 另外随着 在生命早期的发展中，葡萄糖氧化的比例 柠檬酸循环中的二氧化碳和水代谢 糖酵解和磷酸己糖中的丙酮酸和乳酸 路径发生了巨大的变化。 本项目的主要目标 是研究缺氧如何影响 在发育过程中调节大脑葡萄糖代谢。 这个项目 将集中在酶控制各自的通量， 己糖激酶、葡萄糖-6-磷酸脱氢酶和α-酮戊二酸 未成熟和成熟大鼠新皮层脱氢酶复合体 暴露于急性和慢性缺氧期。  我们的一般 一种假说认为，特定代谢调节的差异 有助于维持磷酸化状态的途径 是关键酶活性或其 调制器。 使用体内和体外NMR光谱，MRI和 体外酶测定，我们将不仅检查 葡萄糖通量，所涉及的机制和缺氧对 这些，但也有重要性和作用，这种通量发挥， 未成熟和成熟的神经细胞功能和存活潜力 话题吧

Maintenance of the phosphorylation state of the brain, which is essential for cellular viability and normal brain function, requires an unimpeded supply of glucose and oxygen. Under conditions of oxygen insufficiency, changes in certain modulators (such as ATP, G-6-P, G-1, 6-DP) may enhance the rate of glucose utilization by their action on key enzymes in the respective metabolic pathways. In addition, with development in early life, the proportion of glucose oxidized to carbon dioxide and water in the citric acid cycle to that metabolized to pyruvate and lactate in the glycolytic and hexosemonophosphate pathways changes dramatically. The primary objective of this project is to investigate how hypoxia impacts upon the mechanisms which regulate brain glucose metabolism during development. This project will center on the enzymatic control of the respective fluxes through hexokinase, glucose-6-phosphate dehydrogenase, and alpha-ketoglutarate dehydrogenase complex in the neocortex of immature and mature rats exposed to acute and chronic periods of hypoxia.  Our general hypothesis is that differences in the regulation of specific metabolic pathways contributing to the maintenance of the phosphorylation state are the result of changes in key enzymatic activities or their modulators. Using in-vivo and in-vitro NMR spectroscopy, MRI and enzymatic assays n-vitro, we will examine not only the maturation of glucose flux, the mechanisms involved and the effect of hypoxia on these but also the importance and the role that such fluxes play in nerve cell function and survival potential in the immature and mature subject.