SKELETAL MUSCLE INJURY: ENERGY METAB, MUSCLE PERFUSION & EXERCISE PERFORMANCE

维持大脑的磷酸化状态，这是 对细胞存活和正常大脑功能至关重要，需要 畅通无阻的葡萄糖和氧气供应。在氧气条件下 不足，某些调节剂(如ATP，G-6-P，G-1， 6-DP)可能通过作用于 各自代谢途径中的关键酶。此外，使用 在生命早期的发育中，葡萄糖被氧化成 二氧化碳和水在柠檬酸的循环中代谢 糖酵解和己糖单磷酸盐中的丙酮酸和乳酸 路径发生了戏剧性的变化。这个项目的主要目标是 就是研究低氧如何影响 在发育过程中调节大脑的葡萄糖代谢。这个项目 将集中在通过酶对各自的通量进行控制 己糖激酶、葡萄糖-6-磷酸脱氢酶和α-酮戊二酸 幼年和成年大鼠新皮质中的脱氢酶复合体 暴露于急性和慢性缺氧期。我们的将军 假说是特定新陈代谢调节的差异 有助于维持磷酸化状态的途径 是关键酶活性变化的结果，还是它们的 调制器。利用体内和体外核磁共振波谱、MRI和 酶分析n-体外，我们将不仅检测成熟度 葡萄糖通量、相关机制及低氧对其影响 但也包括这些助熔剂的重要性和作用 未成熟与成熟人群的神经细胞功能及生存潜能 主题。

Maintenance of the phosphorylation state of the brain, which is essential for cellular viability and normal brain function, requires an unimpeded supply of glucose and oxygen. Under conditions of oxygen insufficiency, changes in certain modulators (such as ATP, G-6-P, G-1, 6-DP) may enhance the rate of glucose utilization by their action on key enzymes in the respective metabolic pathways. In addition, with development in early life, the proportion of glucose oxidized to carbon dioxide and water in the citric acid cycle to that metabolized to pyruvate and lactate in the glycolytic and hexosemonophosphate pathways changes dramatically. The primary objective of this project is to investigate how hypoxia impacts upon the mechanisms which regulate brain glucose metabolism during development. This project will center on the enzymatic control of the respective fluxes through hexokinase, glucose-6-phosphate dehydrogenase, and alpha-ketoglutarate dehydrogenase complex in the neocortex of immature and mature rats exposed to acute and chronic periods of hypoxia.  Our general hypothesis is that differences in the regulation of specific metabolic pathways contributing to the maintenance of the phosphorylation state are the result of changes in key enzymatic activities or their modulators. Using in-vivo and in-vitro NMR spectroscopy, MRI and enzymatic assays n-vitro, we will examine not only the maturation of glucose flux, the mechanisms involved and the effect of hypoxia on these but also the importance and the role that such fluxes play in nerve cell function and survival potential in the immature and mature subject.