Catecholamine Aptamers: a Thermodynamic Approach

儿茶酚胺适体：热力学方法

• 批准号: 7056958
• 负责人: JOSHUA E SOKOLOSKI
• 金额: $ 3.35万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7056958
• 关键词: DNA; RNA; catecholamines; oligonucleotides

DESCRIPTION (provided by applicant): Discrimination between dopamine (DA) and norepinephrine (NE) is of considerable interest in modern methods of monitoring neurotransmission. It is proposed that RNA/DNA aptamers may be employed to bind DA or NE with high specificity and sensitivity. This binding interaction would be the basis for an optical or electrochemical based sensing scheme for distinguishing between the two neurotransmitters in biological samples. RNA and DNA aptamers have been shown to bind small molecule ligands with considerable strength and specificity and there has some success with incorporating them into sensor design. This project will start with thermodynamic characterization of a model RNA aptamer that binds the dye malachite green. These thermodynamic criteria for specific binding will then applied to the selection of aptamer candidates for binding DA over NE and/or NE over DA. The long-term goal of the project would be to incorporate the aptameric structures into an in vivo or in vitro biosensor. Such a sensing method would be of great benefit to in vivo studies of diseases and disorders of the central nervous system.

描述（由申请人提供）：多巴胺（DA）和去甲肾上腺素（NE）之间的区别在监测神经传递的现代方法中引起了相当大的兴趣。提出RNA/DNA适体可用于以高特异性和灵敏度结合DA或NE。这种结合相互作用将成为基于光学或电化学的传感方案的基础，用于区分生物样品中的两种神经递质。 RNA 和 DNA 适体已被证明能够以相当大的强度和特异性结合小分子配体，并且将它们纳入传感器设计中取得了一些成功。该项目将从结合孔雀石绿染料的模型 RNA 适体的热力学表征开始。然后将这些特异性结合的热力学标准应用于选择用于结合DA而不是NE和/或NE而不是DA的适体候选者。该项目的长期目标是将适体结构整合到体内或体外生物传感器中。这种传感方法对于中枢神经系统疾病和病症的体内研究将大有裨益。