Catecholamine Aptamers: a Thermodynamic Approach

儿茶酚胺适体：热力学方法

• 批准号: 7342087
• 负责人: JOSHUA E SOKOLOSKI
• 金额: $ 3.35万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7342087
• 关键词: Address; Affinity; Bacillus subtilis; Binding; Biological; Biosensor; Catecholamines; DNA; Detection; Discrimination; Disease; Dopamine; Dyes; Entropy; Exclusion; Fellowship; Goals; Guanine; Hydrogen Bonding; In Vitro; Individual; Ligands; Malachite green; Methods; Modeling; Molecular; Monitor; Muscle Rigidity; Names; Neuraxis; Neurotransmitters; Norepinephrine; Nucleotides; Optics; Performance; RNA; Rate; Role; Sampling; Scheme; Sensitivity and Specificity; Signal Transduction; Solid; Solutions; Specificity; Structure; System; Testing; Thermodynamics; Untranslated Regions; Work; aptamer; base; design; functional group; improved; in vivo; interest; molecular recognition; neurotransmission; research study; sensor; size; small molecule; success; tetramethylrosamine

Discrimination between dopamine (DA) and norepinephrine (NE) is of considerable interest in modern methods of monitoring neurotransmission. It is proposed that RNA/DNA aptamers may be employed to bind DA or NE with high specificity and sensitivity. This binding interaction would be the basis for an optical or electrochemical based sensing scheme for distinguishing between the two neurotransmitters in biological samples. RNA and DNA aptamers have been shown to bind small molecule ligands with considerable strength and specificity and there has some success with incorporating them into sensor design. This project will start with thermodynamic characterization of a model RNA aptamer that binds the dye malachite green. These thermodynamic criteria for specific binding will then applied to the selection of aptamer candidates for binding DA over NE and/or NE over DA. The long-term goal of the project would be to incorporate the aptameric structures into an in vivo or in vitro biosensor. Such a sensing method would be of great benefit to in vivo studies of diseases and disorders of the central nervous system.

多巴胺(DA)和去甲肾上腺素(NE)之间的区别在现代医学中是相当感兴趣的 监测神经传递的方法。有观点认为，RNA/DNA适配子可用于 与DA或NE结合具有很高的特异性和敏感性。这种结合相互作用将是光学 或基于电化学的传感方案来区分两种神经递质 生物样本。RNA和DNA适配子已被证明能与小分子配体结合 相当大的强度和特异性，将它们集成到传感器中取得了一些成功 设计。该项目将从模型RNA适体的热力学特征开始，该模型RNA适配子结合 把孔雀石染成绿色。然后，这些特定结合的热力学标准将应用于选择 NE和/或NE上的DA结合DA的适配子候选。该项目的长期目标是 将适体结构结合到体内或体外生物传感器中。这样一种感测方法 这将对中枢神经系统疾病和紊乱的体内研究大有裨益。

项目成果

Prevalence of syn nucleobases in the active sites of functional RNAs.

• DOI: 10.1261/rna.2759911
• 发表时间: 2011-10
• 期刊: RNA
• 影响因子: 4.5
• 作者: Joshua E. Sokoloski;Stephanie A. Godfrey;Sarah E Dombrowski;P. Bevilacqua

Thermodynamics of ligand binding to a heterogeneous RNA population in the malachite green aptamer.

孔雀绿适体中配体与异质 RNA 群结合的热力学。

• DOI: 10.1021/bi201642p
• 发表时间: 2012
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Sokoloski,JoshuaE;Dombrowski,SarahE;Bevilacqua,PhilipC
• 通讯作者: Bevilacqua,PhilipC