Songbird Neurogenomics Initiative

鸣鸟神经基因组学计划

• 批准号: 7101607
• 负责人: DAVID F CLAYTON
• 金额: $ 31.47万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7101607
• 关键词: Internet; functional /structural genomics; gene expression; genetic regulation; genome; high throughput technology; informatics; interdisciplinary collaboration; learning; microarray technology; molecular biology information system; neurogenetics; nucleic acid sequence; songbirds

DESCRIPTION (provided by applicant): Songbirds offer valuable opportunities for fundamental neuroscience research relevant to human health and disease. The Songbird Neurogenomics (SoNG) Initiative aims to advance this by aggressive, community-level application of emerging technologies in genomics and bioinformatics. In the previous period, more than 40,000 tags for genes expressed in songbird brain (Expressed Sequence Tags, ESTs) were created, from which a DNA microarray was produced for gene expression studies. 20 different research groups submitted proposals for experiments using these microarrays. The first aim now is to follow through with execution of these proposals. Collectively they address a broad range of fundamental research questions about functional relationships between brain and genome. More than 800 brain samples will be analyzed by microarray, following a novel "Community Collaboration" model whereby each individual research group provides brain samples (rigorously documented) to a collaborating core at Illinois, and the core executes all subsequent steps from RNA purification through microarray analysis. With common reference samples and techniques, the resulting expression data can be compared directly across all experiments. To facilitate analysis of results and sharing of data, an integrated, web-based suite of database, bioinformatics and statistical resources will be developed based on the NIH-supported "Beehive" project. Genomic information will be further enhanced through additional EST production, development of a Genome Browser track, and creation of an improved, next-generation microarray. These resources will then be focused to complete a deep profile of brain regions key to song learning, assessing how they compare to other regions including brain areas that correspond to parts of human brain (such as auditory cortex and cortical regions for the control of speech production). A series of community conferences will be held to promote awareness and use of these tools and data, and to focus attention on "next frontiers" for songbird neurogenomics. The songbird brain is of great interest for human neuroscience because it displays an unusual level of organization related to learning, adaptation, gender differences and vocal communication. Fundamental principles worked out first in songbirds have proven true for humans. Moreover, the approach here may serve as a model for doing science on a community scale, embracing individual initiative but coordinating efforts to achieve a larger synergy.

描述（由申请人提供）：鸣禽为与人类健康和疾病相关的基础神经科学研究提供了宝贵的机会。 Songbird 神经基因组学 (SoNG) 计划旨在通过在社区层面积极应用基因组学和生物信息学新兴技术来推动这一目标。 在前一阶段，我们创建了超过40,000个鸣禽大脑表达基因标签（表达序列标签，EST），并从中制作了DNA微阵列用于基因表达研究。 20 个不同的研究小组提交了使用这些微阵列进行实验的提案。 现在的首要目标是落实这些建议。 他们共同解决了有关大脑和基因组之间功能关系的广泛基础研究问题。 超过 800 个大脑样本将通过微阵列进行分析，遵循一种新颖的“社区合作”模式，每个研究小组都向伊利诺伊州的合作核心提供大脑样本（经过严格记录），该核心执行从 RNA 纯化到微阵列分析的所有后续步骤。 利用通用的参考样本和技术，可以在所有实验中直接比较所得的表达数据。 为了促进结果分析和数据共享，将基于 NIH 支持的“Beehive”项目开发一套集成的、基于网络的数据库、生物信息学和统计资源套件。 基因组信息将通过额外的 EST 生产、基因组浏览器轨道的开发以及改进的下一代微阵列的创建来进一步增强。 然后，这些资源将集中用于完成对歌曲学习至关重要的大脑区域的深入分析，评估它们与其他区域的比较，包括与人脑部分相对应的大脑区域（例如听觉皮层和控制言语产生的皮层区域）。 将举行一系列社区会议，以提高对这些工具和数据的认识和使用，并将注意力集中在鸣禽神经基因组学的“下一个前沿”。 鸣禽的大脑对人类神经科学非常感兴趣，因为它表现出与学习、适应、性别差异和声音交流相关的不同寻常的组织水平。 首先在鸣禽中制定的基本原理已被证明适用于人类。 此外，这里的方法可以作为在社区规模上进行科学研究的模型，拥抱个人主动性，但协调努力以实现更大的协同作用。