Multidimensional Protein Identification

描述（由申请人提供）： 我们在斯克里普斯研究所找到了国家癌症研究所资助的研究人员 （TSRI）与研究项目有当前和预期的需求， Proteomics Core.此外，在核心中使用技术将使 对他们的研究有很大的影响。 目前，TSRI没有采用集成的蛋白质组学核心， 解析、显示、定量和表征复杂蛋白质的技术 以高吞吐量的方式进行阵列。为了实现这一未满足的研究资源， 我们建议建立一个机构性的蛋白质组学核心。提供 蛋白质组学的必要工具，核心将提供最先进的 蛋白质分离、解析和鉴定技术。它还将 提供对生物信息学基础设施的访问， 分析、管理、存档和与公共蛋白质数据库的链接。 TSRI机构蛋白质组学核心的建立将提供 所有TSRI研究人员，无论是不是蛋白质组学研究人员， 专家，每天都能接触到尖端技术， 蛋白质的表达、修饰、相互作用或细胞定位。

DESCRIPTION (provided by applicant): We have identified NCI-funded investigators at The Scripps Research Institute (TSRI) with research projects having current and anticipated needs for a Proteomics Core. Moreover, the use of technology in the Core would have considerable impact on their research. Currently, there is no Proteomics Core at TSRI employing integrated technologies to resolve, display, quantify, and characterize complex protein arrays in a high throughput manner. To fulfill this unmet research resource, we are proposing to establish an institutional Proteomics Core. To provide the necessary tools for proteomics, the core will provide state of the art technology for protein isolation, resolution, and identification. It will also provide access to a bioinformatics infrastructure designed to facilitate data analysis, management, archiving, and links to public protein databases. The establishment of a TSRI institutional Proteomics Core would provide resources whereby all TSRI investigators, whom are and are not proteomic specialists, everyday access to cutting-edge technology to assess global expression, modification, interactions or cellular localization of proteins.