Multidimensional Protein Identification

描述(由申请人提供)： 我们已经确认了斯克里普斯研究所由NCI资助的调查人员 (TSRI)具有当前和预期需求的研究项目 蛋白质组学核心。此外，在核心中使用技术将具有 对他们的研究产生了相当大的影响。 目前，TSRI没有采用集成的蛋白质组学核心 复杂蛋白质的解析、显示、量化和表征技术 以高吞吐量的方式创建阵列。为了满足这一未得到满足的研究资源， 我们提议建立一个机构蛋白质组学核心。以提供 蛋白质组学的必要工具，核心将提供最先进的 蛋白质分离、解析和鉴定技术。它还将 提供对旨在促进数据的生物信息学基础设施的访问 分析、管理、存档和到公共蛋白质数据库的链接。 TSRI机构蛋白质组学核心的建立将提供 所有TSRI研究人员，谁是蛋白质组，谁不是蛋白质组的资源 专家，每天都能获得尖端技术来评估全球 蛋白质的表达、修饰、相互作用或细胞定位。

DESCRIPTION (provided by applicant): We have identified NCI-funded investigators at The Scripps Research Institute (TSRI) with research projects having current and anticipated needs for a Proteomics Core. Moreover, the use of technology in the Core would have considerable impact on their research. Currently, there is no Proteomics Core at TSRI employing integrated technologies to resolve, display, quantify, and characterize complex protein arrays in a high throughput manner. To fulfill this unmet research resource, we are proposing to establish an institutional Proteomics Core. To provide the necessary tools for proteomics, the core will provide state of the art technology for protein isolation, resolution, and identification. It will also provide access to a bioinformatics infrastructure designed to facilitate data analysis, management, archiving, and links to public protein databases. The establishment of a TSRI institutional Proteomics Core would provide resources whereby all TSRI investigators, whom are and are not proteomic specialists, everyday access to cutting-edge technology to assess global expression, modification, interactions or cellular localization of proteins.