CORE--BLOOD AND TISSUE SPECIMEN

描述（由申请方提供）：该核心将作为血液和组织研究的重要资源中心，与脑出血和缺血性梗死的积极介入治疗相关，是完成研究项目不可或缺的一部分。它将为来自研究患者的血液和组织的分析带来与中风发病机制相关的形态解剖学技术的丰富专业知识，并为来自研究患者活检和尸检标本的脑组织样本的关键研究者提供访问。核心将支持项目1，MR RESCUE试验，在该试验中，将在治疗性血栓切除术过程中清除血管内血栓物质。该核心将帮助研究者研究血栓形成的生化发病机制及其在脑梗死演变中的可能重要性，并将帮助研究者将其发现与血栓的光学显微镜/超微结构特征相关联。核心还将 为项目2提供支持，即HEME手术试验，在该试验中，来自“凝块”的脑组织 在自发性脑内（实质）出血患者中进行的疏散将仔细评估出血的可能病因和对脑内血液的组织反应，并准备采用定性、实时RT-PCR和cDNA阵列研究差异基因表达。核心将储存适当患者的血液样本用于DNA分析，并在适当审查其研究目标和项目后将其提供给研究人员。该核心将作为一个重要的“血液和脑组织”资源，SPOTRIAS研究人员在加州大学洛杉矶分校，和一个更大的网络的研究人员在治疗急性缺血性和出血性中风的利益。

DESCRIPTION (provided by the applicant): This Core will serve as a vital resource center for blood and tissue-based studies integral to the completion of investigational projects, related to aggressive, interventional treatment of cerebral hemorrhage and ischemic infarcts. It will bring to analyses of blood and tissues derived from study patients a wealth of expertise in morphoanatomical techniques pertinent to stroke pathogenesis, and provide access to key investigators of samples of brain tissue derived from biopsy and autopsy specimens of study patients. The core will support Project 1, the MR RESCUE trial, in which intravascular thrombus material will be removed in the course of therapeutic thrombectomy. The core will assist investigators studying the biochemical pathogenesis of thrombus formation, and its possible importance in evolution of cerebral infarcts, and will assist investigators in correlating their findings with light microscopic/ultrastructural features of the thrombi. The core will also provide support to Project 2, the HEME Surgery trial, in which brain tissue originating from 'clot' evacuations carried out in patients with spontaneous intracerebral (parenchymal) hemorrhage will be carefully evaluated for both the likely etiology of hemorrhage and the tissue response to intracerebral blood, and readied for studies of differential gene expression employing qualitative, real-time RT-PCR and cDNA arrays. The Core will store blood samples from appropriate patients for DNA analysis, and provide these to researchers following appropriate review of their investigational goals and projects. The Core will serve as an important 'blood and brain tissue' resource to SPOTRIAS investigators at UCLA, and a larger network of investigators with interests in the treatment of acute ischemic and hemorrhagic stroke.