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600 MHz Nuclear Magnetic Resonance Spectrometer

600 MHz 核磁共振波谱仪

基本信息

批准号：
7047279
负责人：
DANIEL S SEM
金额：
$ 50万
依托单位：
MARQUETTE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-30 至 2006-09-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7047279
关键词：
NAD(H) phosphate biomedical equipment purchase nuclear magnetic resonance spectroscopy proteomics

项目摘要

DESCRIPTION (provided by applicant): A high field multinuclear NMR spectrometer is needed to support new and ongoing projects at Marquette University and the greater Milwaukee area. The proposed instrument will support and extend existing NIH and other funded projects for 6 major users and 4 minor users from 3 institutions. The major user group is funded by 8 NIH grants, and minor users are funded from various sources. The major users are involved in projects directed to problems in biochemistry that make central use of NMR, and the minor users have projects that rely heavily on NMR in a supporting role. A key feature required of the spectrometer will be the high sensitivity and resolution available at 600 MHz, in order to study protein-ligand interactions. The NMR facility will offer a wide variety of probe choices, including MicroFlow and BB, thereby satisfying a regional need unmet by other facilities. Projects include: (1) Probing protein-ligand interactions in chemical proteomics: NMR methods development and application (Sem), (2) Complementary use of NMR and Raman studies to probe ligand binding to cytochrome P450cam (Kincaid), (3) Role of Su e information of the Fi-Fo ATP synthase dimeric complex: structural and binding studies (Stuart), (4) Mechanism of flagellar radial spoke: ligand binding and structural studies of radial spoke protein 2 (RSP2) (Yang), (5) Structural characterization of molecular motions in NADPH-dependant cytochrome P450 oxidoreductase (Kim) and (6) Substrate binding geometries in Fell dependent non-heme oxygenases from paramagnetic relaxation and heteronudear correlation (Moran). The spectrometer is the center-piece of the "Chemical Proteomics Facility at Marquette", and accordingly has the strong support of Marquette University which has committed the additional $368,945 needed beyond the $500,000 being requested, as well as needed space, facilities, staff, cryogens, warranties and other support. Although neighboring NMR facilities are currently being used, they cannot meet existing demand due largely to the need for travel of > 100 miles and advance scheduling for routine high-field experiments. Such an instrument would therefore accelerate already funded (predominantly NIH) projects across departments and institutions, as well as extend their scope. It will also better leverage the use of regional facilities for follow-up refinement studies, rather than routine early-stage experiments.

描述（由申请人提供）：需要一台高场多核核磁共振波谱仪来支持马奎特大学和大密尔沃基地区的新项目和正在进行的项目。拟议的工具将支持和扩展现有的NIH和其他资助项目，供来自3个机构的6个主要用户和4个次要用户使用。主要用户群由8个NIH赠款资助，次要用户由各种来源资助。主要用户参与了针对生物化学问题的项目，这些项目主要使用NMR，而次要用户的项目严重依赖NMR的支持作用。光谱仪所需的一个关键特性是在600 MHz下的高灵敏度和分辨率，以研究蛋白质-配体相互作用。NMR设施将提供各种探头选择，包括MicroFlow和BB，从而满足其他设施无法满足的区域需求。项目包括：（1）在化学蛋白质组学中探测蛋白质-配体相互作用：NMR方法的开发和应用（Sem），（2）互补使用NMR和拉曼研究来探测配体与细胞色素P450 cam的结合（Kincaid），（3）Fi-Fo ATP合酶二聚体复合物的表面信息的作用：结构和结合研究（Stuart），（4）鞭毛放射状辐条的机制：放射状辐条蛋白2（RSP 2）的配体结合和结构研究（Yang），（5）NADPH依赖性细胞色素P450氧化还原酶中分子运动的结构表征（Kim）和（6）Fell依赖性非血红素加氧酶中顺磁弛豫和异核相关的底物结合几何形状（Moran）。光谱仪是“马奎特化学蛋白质组学设施”的核心，因此得到了马奎特大学的大力支持，该大学承诺在所需的50万美元之外再提供368，945美元，以及所需的空间、设施、工作人员、冷冻剂、保修和其他支持。虽然目前正在使用邻近的NMR设施，但它们无法满足现有的需求，主要是因为需要行程> 100英里，并且需要提前安排常规高场实验。因此，这样的工具将加速跨部门和机构的已资助项目（主要是NIH），并扩大其范围。它还将更好地利用区域设施进行后续改进研究，而不是例行的早期实验。