LASER SCANNING CONFOCAL MICROSCOPE

激光扫描共焦显微镜

• 批准号: 6877422
• 负责人: NIGEL W BUNNETT
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-09 至 2007-05-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877422
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; cell biology; computer program /software; confocal scanning microscopy; protein quantitation /detection

DESCRIPTION (provided by applicant): This proposal is for the purchase of a Zeiss LSM 510 confocal microscope to be used by seven principal investigators and their laboratories in support of eighteen NIH funded projects. These groups will occupy approximately 85% time on the instrument, minor users will have access to the equipment at other times. The requested instrument offers the flexibility of a multi-user microscope that will be the "work horse" for many laboratories. A four-laser microscope is requested (blue diode 405 nm, argon 458, 477, 488 and 514 nm, Hene 543 nm, Hene 633 nm lasers), allowing simultaneous or sequential observation of commonly used blue yellow green, red and far red emitting fluorophores. The instrument will be mounted on a Zeiss 200 Axiovert inverted microscope, equipped with a heated stage motorized in the x, y and z axes. This microscope will provide the flexibility of observation of fixed tissues and cultured cells, as well as observations of dynamic processes in living cells. Objectives are requested to permit the optimal collection of fluorescence signals from fixed tissues and cells, and of cultured cells at an oil/glass/water interface. Software is requested that will permit three-dimensional reconstruction of objects, real time analysis of signal intensities, and sequential collection of z-series from multiple cells in different fields for prolonged periods due to the x/y motorized stage). All of the major users are experienced microscopists, most of whom have previously used confocal microscopy. Existing confocal microscopes in the investigators' laboratories are coming to the end of their useful life and presently require considerable and costly maintenance. Importantly, service contracts will not be offered in the future, requiring availability of a new instrument. Other instruments at UCSF are extensively used by other groups, are not sufficiently flexible for the required uses, or are at other distant campuses. Some of these instruments will be moved to other campuses when faculty relocates to the new UCSF campus at Mission Bay. An impartial faculty committee will oversee use of the microscope. A recharge system will be implemented to fund the routine maintenance and support of the microscope. All users will take a classroom based and hands on training program. Institutional support includes provision of newly refurbished space, funding of technical support for three years, provision of computing support, and fiscal and administrative oversight and support.

描述（由申请人提供）：该提案是为了购买Zeiss LSM 510共聚焦显微镜，可供七名主要研究人员及其实验室使用，以支持18 NIH资助的项目。这些小组将在乐器上占用大约85％的时间，次要用户将在其他时间访问设备。请求的仪器提供了多用户显微镜的灵活性，这将是许多实验室的“工作马”。请求使用四激光显微镜（蓝色二极管405 nm，氩458、477、488和514 nm，Hene 543 nm，Hene 633 nm激光器），允许同时或顺序观察常用的蓝色绿色，红色绿色，红色和远红色的红色和远红色的荧光荧光片。该仪器将安装在Zeiss 200 Axiovert倒置显微镜上，该显微镜配备了在X，Y和Z轴上机动的加热阶段。该显微镜将提供观察固定组织和培养细胞的灵活性，以及​​对活细胞中动态过程的观察。要求目标允许从固定组织和细胞以及油/玻璃/水界面的培养细胞中最佳收集荧光信号。 请求软件，该软件将允许对象的三维重建，信号强度的实时分析以及由于X/Y机动阶段而导致的延长时期内从不同场中的多个单元中收集Z系列）。所有主要用户都是经验丰富的显微镜，其中大多数以前都使用了共聚焦显微镜。研究人员实验室中的现有共聚焦显微镜即将到来，目前需要大量且昂贵的维护。重要的是，将来将不提供服务合同，需要新工具的可用性。 UCSF的其他仪器被其他组广泛使用，不足以适合所需用途，或者在其他遥远的校园中。当教职员工搬迁到Mission Bay的新UCSF校园时，其中一些乐器将移至其他校园。公正的教师委员会将监督显微镜的使用。将实施一个充电系统，以资助显微镜的常规维护和支持。所有用户都将进行基于教室和动手培训计划。机构支持包括提供新的翻新空间，三年的技术支持资金，提供计算支持以及财政和行政监督和支持。