LTQ MS for High Sensitivity (Phospho) Proteomics

描述（由申请人提供）：13名美国国立卫生研究院资助的研究人员申请了一台Finnigan LTQ线性离子阱质谱仪，该质谱仪配备了完整的Dionex UltiMate纳米- lc系统，纳米ESI源和BioWorks/TurboSEQUEST/SALSA数据分析工作站，用于高灵敏度蛋白质组学和翻译后修饰分析。与我们目前的LCQ DECA相比，LTQ的灵敏度提高了10倍，从而大大提高了实现拟议研究目标的潜力。在大奥尔巴尼地区没有其他LTQ存在，也没有任何仪器具有MS/MS敏感性的蛋白质组学或LC-MS/MS/MS能力的肽磷酸化分析。LTQ的授予将使许多项目的蛋白质组学分析远远超出我们目前的灵敏度限制。具体研究领域包括：人呼吸道细胞色素P450酶的调控/修饰；致不孕症的人卵巢自身免疫抗原的鉴定结核分枝杆菌和鼠疫耶尔森菌生物学；metal-induced免疫病理;与人溶酶体蛋白表达及甲氨蝶呤耐药性有关。翻译后修饰和磷酸化测定项目包括：spisella来源的中心体复合物蛋白；运动蛋白，动力蛋白；人血管平滑肌蛋白；人类病原体密螺旋体运动蛋白；结肠癌中的生长因子和信号转导受体。

DESCRIPTION (provided by applicant): Thirteen NIH-funded investigators request funds for a Finnigan LTQ linear ion trap MS, equipped with a complete Dionex UltiMate nano-LC system, a nano- ESI source, and a data analysis workstation with BioWorks/TurboSEQUEST/SALSA, for high sensitivity proteomics and post-translational modification analyses. The potential to achieve proposed research goals will be substantially enhanced by the 10-fold sensitivity increase of the LTQ compared to our current LCQ DECA. No other LTQ exists in the greater Albany area, nor is there any instrument with the MS/MS sensitivity for proteomics or LC-MS/MS/MS capability for peptide phosphorylation analyses. Award of the LTQ will permit proteomics analyses on many projects that are well beyond our current sensitivity limits. Specific research areas include: regulation/ modification of cytochrome P450 enzymes in the human respiratory tract; identification of human ovarian autoimmune antigens causing infertility; M. tuberculosis and Yersinia pestis biology; metal-induced immunopathology; and human lysosomal protein expression and methotrexate resistance. Post-translational modification and phosphorylation determination projects include: Spisula-derived centrosome complex proteins; the motor protein, dynein; human vascular smooth muscle proteins; motility proteins of the human pathogen, Treponema denticola; and growth factors and signal transduction receptors in colon cancers. Currently, all listed co-investigators collaborate with the Wadsworth Mass Spectrometry/Proteomics Core. The Core Director (the Principal Investigator) has extensive experience in protein mass spectrometry and the management of a multi-instrument, multi-user facility. The LTQ MS will be integrated into this core, which has a record of both routine analyses and research collaborations with investigators at Albany Medical College, SUNY Albany, Rensselaer Polytechnic Institute, and Roswell Park and will benefit other NIH-funded research programs at these institutions.