LTQ MS for High Sensitivity (Phospho) Proteomics

用于高灵敏度（磷酸化）蛋白质组学的 LTQ MS

• 批准号: 6876395
• 负责人: CHARLES R HAUER
• 金额: $ 39.96万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-15 至 2006-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876395
• 关键词: biomedical equipment purchase; computer data analysis; electrospray ionization mass spectrometry; posttranslational modifications; proteomics

DESCRIPTION (provided by applicant): Thirteen NIH-funded investigators request funds for a Finnigan LTQ linear ion trap MS, equipped with a complete Dionex UltiMate nano-LC system, a nano- ESI source, and a data analysis workstation with BioWorks/TurboSEQUEST/SALSA, for high sensitivity proteomics and post-translational modification analyses. The potential to achieve proposed research goals will be substantially enhanced by the 10-fold sensitivity increase of the LTQ compared to our current LCQ DECA. No other LTQ exists in the greater Albany area, nor is there any instrument with the MS/MS sensitivity for proteomics or LC-MS/MS/MS capability for peptide phosphorylation analyses. Award of the LTQ will permit proteomics analyses on many projects that are well beyond our current sensitivity limits. Specific research areas include: regulation/ modification of cytochrome P450 enzymes in the human respiratory tract; identification of human ovarian autoimmune antigens causing infertility; M. tuberculosis and Yersinia pestis biology; metal-induced immunopathology; and human lysosomal protein expression and methotrexate resistance. Post-translational modification and phosphorylation determination projects include: Spisula-derived centrosome complex proteins; the motor protein, dynein; human vascular smooth muscle proteins; motility proteins of the human pathogen, Treponema denticola; and growth factors and signal transduction receptors in colon cancers. Currently, all listed co-investigators collaborate with the Wadsworth Mass Spectrometry/Proteomics Core. The Core Director (the Principal Investigator) has extensive experience in protein mass spectrometry and the management of a multi-instrument, multi-user facility. The LTQ MS will be integrated into this core, which has a record of both routine analyses and research collaborations with investigators at Albany Medical College, SUNY Albany, Rensselaer Polytechnic Institute, and Roswell Park and will benefit other NIH-funded research programs at these institutions.

DESCRIPTION (provided by applicant): Thirteen NIH-funded investigators request funds for a Finnigan LTQ linear ion trap MS, equipped with a complete Dionex UltiMate nano-LC system, a nano- ESI source, and a data analysis workstation with BioWorks/TurboSEQUEST/SALSA, for high sensitivity proteomics and post-translational modification analyses.与我们当前的LCQ DECA相比，LTQ的10倍灵敏度提高将大大提高实现提出的研究目标的潜力。大奥尔巴尼地区没有其他LTQ，也没有任何具有对蛋白质组学或LC-MS/MS/MS/MS/MS/MS/MS/MS/MS磷酸化分析的仪器。 LTQ的奖励将允许对许多超出我们当前敏感性限制的项目进行蛋白质组学分析。特定的研究领域包括：人类呼吸道中细胞色素P450酶的调节/修饰；鉴定人卵巢自身免疫抗原，导致不育症；结核病和耶尔森氏菌生物学；金属诱导的免疫病理学；人类溶酶体蛋白表达和甲氨蝶呤的抗性。翻译后修饰和磷酸化测定项目包括：尖齿的中心体复合蛋白；运动蛋白，动力蛋白；人血管平滑肌蛋白；人类病原体的运动蛋白，treponema denticola;结肠癌中的生长因子和信号转导受体。 目前，所有列出的共同投资者都与Wadsworth质谱/蛋白质组学核心合作。核心主管（主要研究者）在蛋白质质谱和多功能仪器多用户设施的管理方面具有丰富的经验。 LTQ MS将集成到该核心中，该核心与奥尔巴尼医学院，纽约州立大学奥尔巴尼，伦斯勒理工学院和罗斯威尔公园的调查人员进行了常规分析和研究合作的记录，并将使这些机构的其他NIH资助的研究计划受益。