NANO ESI ION TRAP MASS SPEC FOR PROTEOMINCS & MS(N)

13名研究人员正在申请资金，用于配备电喷雾、纳米电喷雾和APCI（大气压化学电离）离子源的离子阱质谱仪（MS）。离子阱MS（奥尔巴尼研究界没有）将用于高灵敏度LC-MS/MS、肽序列、蛋白质组学和多级碰撞诱导解离[MS（n）]分析。这些研究人员由10个NIH和3个NSF资助赠款将直接受益于离子阱MS的使用。其中7个研究人员将使用高灵敏度MS/MS，与自动数据库搜索接口，快速识别肽/蛋白质序列，即蛋白质组学。具体研究领域包括：金属诱导的免疫病理学，M。结核病与巨噬细胞的相互作用，线粒体在细胞凋亡中的作用，跨膜蛋白易位，血管生物学，古细菌中的应激基因调控，有丝分裂相关蛋白，以及PCB在人类乳腺细胞中的雌激素效应。其中六名研究人员将使用MS（N）分析进行分子结构解析，具体而言;不寻常的寡糖、肽二硫键分配、来自海洋生物粘合剂的广泛交联的肽/蛋白质、代谢物分析、复杂的有机地下水污染物和辐射诱导的DNA修饰。所有合作研究人员目前与沃兹沃斯中心的生物质谱核心合作。主要研究者（核心负责人）在生物质谱和多仪器、多用户设施管理方面拥有丰富的经验。离子阱MS将集成到该核心中。沃兹沃斯中心拥有大奥尔巴尼地区唯一的生物MS核心设施，它与奥尔巴尼医学院、纽约州立大学奥尔巴尼和伦斯勒理工学院的研究人员进行常规分析和研究合作，并建立了记录。该仪器的授予将为研究人员提供唯一的自动MS/MS蛋白质组数据库搜索和MS（n）分析的本地访问。在这个时候，我们要求Finnigan LCQ Deca，与Seaquest数据库搜索程序，并配备了Protana纳米电喷雾离子源，作为最适合这些不同应用的仪器。

Thirteen investigators are requesting funds for an ion trap mass spectrometer (MS) equipped with electro spray, nanoelectrospray, and APCI (atmospheric pressure chemical ionization) ion sources. The ion trap MS, which is not available in the Albany research community, will be used for high sensitivity LC-MS/MS, peptide sequence, proteomics and multiple stage collision induced dissociation [MS(n)] analysis. These investigators are funded by 10 NIH and 3 NSF grants that will benefit directly from use of an ion trap MS. Seven of the investigators will use high sensitivity MS/MS interfaced to automated database searches for rapid identification of peptide/protein sequence, i.e. proteomics. Specific research areas include: metal-induced immunopathology, M. tuberculosis interaction with macrophages, the role of mitochondria in apoptosis, protein translocation across membranes, vascular biology, stress gene regulation in archea, mitosis associated proteins, and PCB estrogenic effects in human breast cells. Six of the investigators will use MS (N) analysis for molecular structure elucidation, specifically; unusual oligosaccharides, peptide disulfide bridge assignment, extensively cross- linked peptides/proteins from a marine organism adhesive, metabolite analysis, complex organic ground water pollutants, and radiation-induced DNA modifications. All co-investigators collaborate currently with the Biological Mass Spectrometry Core at the Wadsworth Center. The Principal Investigator (Director of the Core) has extensive experience in biological mass spectrometry and the management of a multi-instrument, multi-user facility. The ion trap MS will be integrated into this core. The Wadsworth Center houses the only biological MS core facility in the greater Albany area, and it has an established record of both routine analysis and research collaboration with investigators at Albany Medical College, SUNY Albany, and Rensselaer Polytechnic Institute. Award of this instrument will afford the only local access of automated MS/MS proteomic data base searching and MS(n) analysis for the investigators. At this time we request a Finnigan LCQ Deca, with the Seaquest database search program, and equipped with a Protana nanoelectrospray ion source, as the instrument best suited to these diverse applications.