Shared Instrument: Focus microPET

共享仪器：Focus microPET

• 批准号: 6877589
• 负责人: Steven Mark Larson
• 金额: $ 49.9万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-15 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877589
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; biomedical equipment resource; positron emission tomography

DESCRIPTION (provided by applicant): The commercially available Focus is a dedicated ultra-high-resolution small-animal 3D positron emission tomograph (PET) (Concorde Microsystems) employing lutetium ortho-oxysilicate (LSO) detector blocks. The LSO blocks in the Focus are unique in that the spacing between adjacent crystal elements (approximately 1x1 mm) is significantly narrower than in other systems, yielding significantly improved spatial resolution approximately- 1 mm (volume resolution: 1 mm3 - 10-fold better than current-generation microPETs). We have had 3 years experience with Concorde's older-design R4 microPET -resulting in 23 publications and 25 abstracts. Although very useful, the R4 has serious limitations for quantification in mouse tumor models (xenograft, transgenic, and knock-out). Our microPET users will thus greatly benefit from the state-of-the-art capabilities offered by the Focus - besides providing badly needed additional microPET imaging time for our many funded studies that involve imaging of radiotracers. We believe that we are well suited by experience and expertise to fully exploit the capabilities of the Focus. In addition, a well-established infrastructure is already in place to provide and coordinate the necessary logistical, technical, and scientific components critical to a successful small-animal imaging program. For example, the Cyclotron and Radiochemistry Core Laboratory includes our new (but fully operational) dual-beam, 19/9 Ebco cyclotron, producing a broad array of positron-emitting radionuclides (eg carbon-11, fluorine-18, gallium-66, yttium-86, iodine-124 etc) and radiotracers (e.g. C11-colchicine and -methionine, F18-FMiso, -FEAU, and -FDHT, Ga66-DOTATOC, Y86-DOTA-Biotin, and I124-FIAU and IAZG). Using such multiple radiotracers, regional tumor perfusion, metabolism, receptor and antigen levels, hypoxia, and gene expression can be assessed serially by microPET in the same animal - prior to, during, and after treatment, including the many novel drug, radiation, and radionuclide therapies being developed at MSKCC. In summary, the Animal Imaging Core Facility at MSKCC is being utilized by a continually expanding number of funded investigators and molecular imaging has emerged as a critical component of our research armamentarium. Acquisition of the state-of-the-art Focus will be an invaluable adjunct to resources already in place, and will further enhance our Center-wide, mission-critical capabilities in molecular imaging.

描述（由申请人提供）： 市售 Focus 是一款专用的超高分辨率小动物 3D 正电子发射断层扫描仪 (PET)（Concorde Microsystems），采用原硅酸镥 (LSO) 探测器块。 Focus 中的 LSO 模块的独特之处在于，相邻晶体元件之间的间距（约 1x1 毫米）明显比其他系统窄，空间分辨率显着提高约 1 毫米（体积分辨率：1 毫米 - 比当前一代 microPET 好 10 倍）。我们在 Concorde 旧式设计的 R4 microPET 方面拥有 3 年经验，已发表 23 篇出版物和 25 篇摘要。尽管 R4 非常有用，但它在小鼠肿瘤模型（异种移植、转基因和敲除）中的定量具有严重的局限性。因此，我们的 microPET 用户将极大地受益于 Focus 提供的最先进的功能 - 除了为我们许多涉及放射性示踪剂成像的资助研究提供急需的额外 microPET 成像时间之外。我们相信，我们的经验和专业知识非常适合充分利用福克斯的能力。此外，完善的基础设施已经到位，可以提供和协调对成功的小动物成像项目至关重要的必要的后勤、技术和科学组成部分。例如，回旋加速器和放射化学核心实验室包括我们新型（但全面运行）双束 19/9 Ebco 回旋加速器，可生产多种正电子发射放射性核素（例如碳 11、氟 18、镓 66、钇 86、碘 124 等）和放射性示踪剂（例如 C11-秋水仙碱和 -蛋氨酸， F18-FMiso、-FEAU 和 -FDHT、Ga66-DOTATOC、Y86-DOTA-生物素、I124-FIAU 和 IAZG)。使用此类多种放射性示踪剂，可以通过 microPET 在同一动物中连续评估区域肿瘤灌注、代谢、受体和抗原水平、缺氧和基因表达 - 在治疗之前、期间和之后，包括 MSKCC 正在开发的许多新型药物、放射和放射性核素疗法。总之，MSKCC 的动物成像核心设施正在被越来越多的受资助研究人员使用，分子成像已成为我们研究装备的重要组成部分。收购最先进的 Focus 将成为对现有资源的宝贵补充，并将进一步增强我们中心在分子成像方面的关键任务能力。

项目成果

PET Imaging of Extracellular pH in Tumors with (64)Cu- and (18)F-Labeled pHLIP Peptides: A Structure-Activity Optimization Study.

• DOI: 10.1021/acs.bioconjchem.6b00306
• 发表时间: 2016-09-21
• 期刊: BIOCONJUGATE CHEMISTRY
• 影响因子: 4.7
• 作者: Demoin, Dustin Wayne;Wyatt, Linden C.;Edwards, Kimberly J.;Abdel-Atti, Dalya;Sarparanta, Mirkka;Pourat, Jacob;Longo, Valerie A.;Carlin, Sean D.;Engelman, Donald M.;Andreev, Oleg A.;Reshetnyak, Yana K.;Viola-Villegas, Nerissa;Lewis, Jason S.
• 通讯作者: Lewis, Jason S.

Annotating STEAP1 regulation in prostate cancer with 89Zr immuno-PET.

• DOI: 10.2967/jnumed.114.145185
• 发表时间: 2014-12
• 期刊: Journal of nuclear medicine : official publication, Society of Nuclear Medicine
• 作者: Doran MG;Watson PA;Cheal SM;Spratt DE;Wongvipat J;Steckler JM;Carrasquillo JA;Evans MJ;Lewis JS
• 通讯作者: Lewis JS

In vivo imaging of GLP-1R with a targeted bimodal PET/fluorescence imaging agent.

• DOI: 10.1021/bc500178d
• 发表时间: 2014-07-16
• 期刊: BIOCONJUGATE CHEMISTRY
• 影响因子: 4.7
• 作者: Brand, Christian;Abdel-Atti, Dalya;Zhang, Yachao;Carlin, Sean;Clardy, Susan M.;Keliher, Edmund J.;Weber, Wolfgang A.;Lewis, Jason S.;Reiner, Thomas
• 通讯作者: Reiner, Thomas

Cabozantinib resolves bone scans in tumor-naïve mice harboring skeletal injuries.

卡博替尼可以解决患有骨骼损伤的未患肿瘤小鼠的骨扫描问题。

• DOI: 10.2310/7290.2014.00026
• 发表时间: 2014
• 期刊: Molecular imaging
• 影响因子: 2.8
• 作者: Doran,MichaelG;Spratt,DanielE;Wongvipat,John;Ulmert,David;Carver,BrettS;Sawyers,CharlesL;Evans,MichaelJ
• 通讯作者: Evans,MichaelJ

Synthesis and Evaluation of a Novel 64Cu- and 67Ga-Labeled Neurokinin 1 Receptor Antagonist for in Vivo Targeting of NK1R-Positive Tumor Xenografts.

用于体内靶向 NK1R 阳性肿瘤异种移植物的新型 64Cu 和 67Ga 标记神经激肽 1 受体拮抗剂的合成和评估。

• DOI: 10.1021/acs.bioconjchem.8b00063
• 发表时间: 2018
• 期刊: Bioconjugate chemistry
• 影响因子: 4.7
• 作者: Zhang,Hanwen;Kanduluru,AnandaKumar;Desai,Pooja;Ahad,Afruja;Carlin,Sean;Tandon,Nidhi;Weber,WolfgangA;Low,PhilipS
• 通讯作者: Low,PhilipS