A Study of Metal Sequestering and Release from Microbes

我们提出的研究将检验我们提出的假设，即微生物多聚磷体(PPbs)是金属隔离的主要场所。我们将使用我们的具有扫描传输模式(STGM)的透射电子显微镜(TEM)和相关的能量色散光谱仪(EDX)来确定哪个细胞 组件隔离了最多的金属。用STEM的光斑模式设置从不同的电池组件获得的光谱将得到。待分析的细胞成分为被膜(鞘)细胞壁、细胞质和PPBs。我们将使用适用于形状的数学公式来计算每个这些组件所具有的细胞的体积。的数量。 每个单元区域中的金属将使用普林斯顿伽马技术IMIX-EDX进行定量测定。根据这些数据，我们将计算每个单元区或组件中隔离的金属量。我们建议使用：(1)波利扁藻，(2)聚球藻，(3)阿尔卑斯卡波拉，(4)铜绿假单胞菌，(5)大肠杆菌，(6)葡萄球菌，(7)枯草芽孢杆菌，(8)醋酸钙不动杆菌，(9)球形节杆菌，(10)酿酒酵母，(11)红酵母。如果我们的假设成立，我们将开发一种去除金属的系统，在该系统中，含有大量PPB的细胞被包裹在海藻酸钠中。好氧系统将转换为厌氧环境。PPBS应在释放金属的同时拆除 变成了上流社会。这应该会让金属被多次浓缩。然后，金属和磷就可以沉淀出来。

We propose research which will test the hypothesis where we propose that microbial polyphosphate bodies (PPBs) are the main sites of metal sequestering. We will use our technology of a transmission electron microscope (TEM) with a scanning transmission mode (STGM) and an associated energy dispersive spectrometer (EDX) to determine which cell component sequesters the most metal. Spectra obtained with the spot mode setting of the STEM from different cell components will be obtained. The cell components to be analyzed are capsule (sheath) cell wall, cytoplasm and PPBs. We will calculate the volume of the cell each of these components has using the appropriate mathematical formula for the shape. The amount of the metal in each cell area will be determined quantitatively using the Princeton Gamma Tech IMIX-EDX. From this data we will calculate the amount of metal sequestered in each cell area or component. We propose using for test organisms: (1) Plectonema boryanum, (2) Synechococcus leopoliensis, (3) Gloeocapsa alpicola, (4) Pseudomonas aeruginosa, (5) Escherichia coli, (6) Staphlococcus areus, (7) Bacillus subtilis, (8) Acinetobacter calcoaceticus, (9) Arthrobacter globiformis, (10) Saccharomyces cervisae, and (11) Rhodotorula rubra. If our hypothesis is sustained we will develop a metal removing system where cells with large numbers of PPBs are enrobed in Na-alginate. The aerobic system will be converted to an anaerobic environment. The PPBs should be dismantled with the metal being released into the supemant. This should allow the metal to be concentrated many times. The metals and the phosphorus can then be precipated.