Neurogenetics of Drosophila reproduction

果蝇繁殖的神经遗传学

• 批准号: 6987146
• 负责人: JEFFREY C HALL
• 金额: $ 26.32万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6987146
• 关键词: Drosophilidae; auditory stimulus; behavioral /social science research tag; behavioral genetics; biological clocks; calcium channel; ethology; gene expression; gene interaction; genetic mapping; genetically modified animals; membrane channels; metamorphosis; mutant; nerve; neurogenetics; point mutation; posttranscriptional RNA processing; reproduction; serotonin; sex behavior; sex determination; tissue mosaicism; transcription factor

DESCRIPTION (provided by applicant): Neurogenetic and molecular neurobiological investigations of sex-specific behavior in Drosophila are proposed. These studies will focus on two of the most salient, quantifiable, and behaviorally important features of the fixed action patterns that define these phenomena: the male's species-specific courtship song and separate components of mating itself. Among the experiments proposed are those involving manipulation of genes that function within the sex-determination hierarchy (SDH) of Drosophila melanogaster: fruitless (fru) and doublesex (dsx). The fru gene will be manipulated to delve into the neural substrates of courtship song, which cannot be performed by fruitless mutants lacking male-specific FRU protein in their ventral nerve cord (VNC); and of mating biology, two elements of which are dramatically perturbed in certain fru mutants. For the other SDH factor (dsx), similar transgene-based experiments are proposed to study the manner by which doublesex participates in programming VNC development and (potentially) regulating its ongoing function--both in the context of a sharply defined abnormality of singing behavior in dsx mutants. The doublesex experiments will be performed against a backdrop of an all-too-common view that sexual differentiation of Drosophila's nervous system is not influenced by this gene--which is belied by the behavioral phenomenon just referred to, along with a recent finding that DSX proteins are found in distinctive patterns within the metamorphosing and mature VNC. With respect to distal genic effectors of courtship actions, it is proposed to assess the effects of a fruitless-associated neurochemical factor (serotonin, or 5HT) that is strongly surmised to coordinate a key feature of mating, along with analyzing genes encoding 5HT-synthetic enzymes that are candidates to be controlled by the FRU transcription factor; and to manipuate neural expression of a song-controlling ion-channel gene (slowpoke). These SDH and effector-related experiments will involve applications of extant mutants (including those recently induced); genetic mosaics; and both spatial plus temporal manipulations of these genes' expressions, via utilization of existing transgenes and creation of novel molecularly engineered, reproductive-related factors, including those cloned from different Drosophila species and bioassayed in D. melanogaster

描述（由申请人提供）：提出了果蝇中性别特异性行为的神经遗传学和分子神经生物学研究。这些研究将集中在定义这些现象的固定动作模式的两个最显着，最量化和行为上重要的特征：男性特定于物种的求爱歌曲和交配本身的单独组成部分。在提出的实验中，有涉及果蝇果蝇（Drosophila Melanogaster）在性别确定层次结构（SDH）中的基因操纵的实验：毫无结果（FRU）和Doublesex（DSX）。 FRU基因将被操纵，以深入研究求爱歌曲的神经底物，这是无法通过缺乏男性特异性FRU蛋白（VNC）的无果突变体（VNC）进行的；以及交配生物学，其中两个元素在某些FRU突变体中剧烈扰动。对于其他SDH因子（DSX），提出了类似的基于转基因的实验来研究Doublesex参与编程VNC开发的方式，并（可能）（可能）调节其持续的功能 - 在DSX突变体中唱歌行为的明确定义的异常情况下。 Doublesex实验将在一个非常常见的观点的背景下进行，即果蝇神经系统的性差异不受该基因的影响 - 该基因被所指的行为现象所掩盖，而最近发现的发现DSX蛋白在中等变形和Mature vnc中被发现了DSX蛋白。关于求爱行动的远端遗传效应因素，建议评估毫无结果相关的神经化学因子（5-羟色胺或5HT）的影响，该因素强烈推测以协调交配的关键特征，并分析编码5HT合成酶的基因，这些基因是候选fru fru fru fru fru fru fru;并操纵歌曲控制的离子通道基因的神经表达（SlowPoke）。这些SDH和与效应相关的实验将涉及现存突变体的应用（包括最近引起的突变体）；遗传马赛克；以及通过利用现有转基因和创建新型分子设计的，生殖相关的因子，包括从不同的果蝇种类克隆的新型转基因并在D. melanogaster中产生生物测定的因子，包括新型分子工程，生殖相关因素，这都是通过使用现有转基因和创建新型分子设计的新型转基因和创建新型分子设计的因子，并在D. melanogaster中产生新型基因的表达式的时空操作，这两者都是空间加上这些基因表达式的时空操作，这两者都是空间加上时间操作，包括从不同的果蝇中的新颖的，以及生物酶生物化合物）