POLARIZED CELL SHAPE CHANGES IN XENOPUS GASTRULATION

爪蟾原肠形成时极化细胞形状的变化

• 批准号: 7156090
• 负责人: Jen-Yi Lee
• 金额: $ 4.6万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-05 至 2009-09-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7156090
• 关键词: Xenopus; actin binding protein; affinity chromatography; affinity labeling; cell cell interaction; cell differentiation; cell growth regulation; cell morphology; cellular polarity; developmental genetics; embryogenic cleavage; guanosinetriphosphatases; histogenesis; immunoprecipitation; nonmammalian vertebrate embryology; postdoctoral investigator; protein quantitation /detection; protein structure function

DESCRIPTION (provided by applicant): My broad research goal is to investigate the cellular mechanisms that drive cell shape changes, and to understand how genetic control of cell fate translates into changes in cell behavior. During the initial stages of gastrulation in the amphibian Xenopus laevis, bottle cells initiate the blastopore lip, creating a crevice for the gastrulating cells to internalize. The shape change in bottle cells is driven by apical constriction, a mechanism that often precedes ingression and invagination. Despite its role in diverse morphogenetic processes, very little is known about the mechanisms responsible for apical constriction. My goal is to establish X. laevis bottle cell formation as a model for investigating apical constriction by examining the cytoskeletal and molecular basis for polarized cell shape changes. In addition, I will identify proteins that are required for apical constriction. In the long term, I will expand my research into X. tropicalis to enable the use of genetic tools to study vertebrate morphogenesis.

描述（由申请人提供）：我广泛的研究目标是研究驱动细胞形状变化的细胞机制，并了解细胞命运的遗传控制如何转化为细胞行为的变化。在两栖动物非洲爪蟾原肠胚形成的最初阶段，瓶状细胞启动了囊胚孔唇，为原肠胚细胞的内化创造了一个缝隙。瓶状细胞的形状变化是由顶端收缩驱动的，这是一种通常先于侵入和内陷的机制。尽管它在不同的形态发生过程中的作用，很少有人知道的机制负责顶端收缩。我的目标是建立X。通过检查极化细胞形状变化的细胞骨架和分子基础，将泡状细胞形成作为研究顶端收缩的模型。此外，我将确定蛋白质所需的顶端收缩。从长远来看，我将把我的研究扩展到X。tropicalis，使使用遗传工具来研究脊椎动物形态发生。