POLARIZED CELL SHAPE CHANGES IN XENOPUS GASTRULATION

爪蟾原肠形成时极化细胞形状的变化

• 批准号: 7286357
• 负责人: Jen-Yi Lee
• 金额: $ 4.88万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-05 至 2009-09-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7286357
• 关键词: Actin-Binding Protein; Actins; Affinity; Affinity Chromatography; Amphibia; Apical; Behavior; Binding; Biological Assay; Blastopores; Cell Communication; Cell Fate Control; Cell Shape; Cells; Complex; Constriction procedure; Depth; Exhibits; F-Actin; Fellowship; Genetic; Goals; Individual; Laboratories; Lip structure; Localized; Measures; Modeling; Molecular; Monomeric GTP-Binding Proteins; Morphogenesis; Names; Non - mammalian blastula; Play; Process; Property; Protein Overexpression; Proteins; Research; Role; Shapes; Staging; Testing; Time; Translating; Xenopus; Xenopus laevis; base; blastocyst; cell behavior; moesin; polarized cell; protein distribution; protein function; tool

DESCRIPTION (provided by applicant): My broad research goal is to investigate the cellular mechanisms that drive cell shape changes, and to understand how genetic control of cell fate translates into changes in cell behavior. During the initial stages of gastrulation in the amphibian Xenopus laevis, bottle cells initiate the blastopore lip, creating a crevice for the gastrulating cells to internalize. The shape change in bottle cells is driven by apical constriction, a mechanism that often precedes ingression and invagination. Despite its role in diverse morphogenetic processes, very little is known about the mechanisms responsible for apical constriction. My goal is to establish X. laevis bottle cell formation as a model for investigating apical constriction by examining the cytoskeletal and molecular basis for polarized cell shape changes. In addition, I will identify proteins that are required for apical constriction. In the long term, I will expand my research into X. tropicalis to enable the use of genetic tools to study vertebrate morphogenesis.

描述（由申请人提供）：我的广泛研究目标是研究驱动细胞形状变化的细胞机制，并了解细胞命运的遗传控制如何转化为细胞行为的变化。在两栖动物非洲爪蟾的原肠胚形成的初始阶段，瓶细胞启动胚孔唇，为原肠胚形成细胞内化创造一个缝隙。瓶细胞的形状变化是由顶端收缩驱动的，这种机制通常发生在进入和内陷之前。尽管它在不同的形态发生过程中发挥着作用，但我们对顶端收缩的机制知之甚少。我的目标是通过检查极化细胞形状变化的细胞骨架和分子基础，建立非洲滑鼠瓶细胞形成模型，作为研究顶端收缩的模型。此外，我将确定顶端收缩所需的蛋白质。从长远来看，我将把我的研究扩展到热带 X. ，以便能够使用遗传工具来研究脊椎动物的形态发生。