Differential expression of collagen by TGF-b1 & b3

TGF-b1对胶原蛋白的差异表达

• 批准号: 7158819
• 负责人: Stephanie R Goldberg
• 金额: $ 5.04万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7158819
• 关键词: biological signal transduction; fibroblasts; gene expression; genetic regulation; phosphorylation; polymerase chain reaction; postdoctoral investigator; procollagen; protein isoforms; scars; transcription factor; transforming growth factors; western blottings; wound healing

DESCRIPTION (provided by applicant): Excess wound contraction and inadequate scar formation in the adult are characterized by collagen deposition resulting in numerous pathologic conditions such as intestinal adhesions and pressure ulcers, for which there are few therapeutic options. In contrast, fetal wounds heal with scarlessly and with a higher ratio of collagen type III to type 1a1 deposition than in adult wounds. Transforming growth factor beta (TGF-b) has been implicated to be an important mediator of wound healing and the TGF-b isoforms may have a role in both the transition from the fetal scarless wound to the adult scar-forming wound. This study aims at exploring the relationship between TGF-b and wound healing by determining if TGF-b1 and -b3 differentially regulate procollagen 1a1 and procollagen III gene expression in an in vitro model by using the same cell- surface receptor yet through different cell signaling pathways.

描述（由申请人提供）：成人伤口过度收缩和疤痕形成不足的特点是胶原蛋白沉积，导致多种病理状况，例如肠粘连和压疮，对此几乎没有治疗选择。相比之下，胎儿伤口愈合后无疤痕，并且与成人伤口相比，III 型胶原蛋白与 1a1 型胶原蛋白沉积的比例更高。转化生长因子β (TGF-b) 被认为是伤口愈合的重要介质，并且 TGF-b 亚型可能在从胎儿无疤痕伤口到成人疤痕形成伤口的转变中发挥作用。本研究旨在通过使用相同的细胞表面受体但通过不同的细胞信号传导途径确定体外模型中 TGF-b1 和 -b3 是否差异调节前胶原 1a1 和前胶原 III 基因表达，探讨 TGF-b 与伤口愈合之间的关系。