Heat Shock Factor Function in Spermatogenesis

热休克因子在精子发生中的作用

• 批准号: 7058072
• 负责人: Donald C. Wilkerson
• 金额: $ 5.04万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7058072
• 关键词: DNA binding protein; cell cycle; chromatin immunoprecipitation; deoxyribonuclease I; developmental genetics; enzyme activity; fertility; gel mobility shift assay; genetic promoter element; genetic regulation; heat shock proteins; immunofluorescence technique; laboratory mouse; meiosis; phosphoprotein phosphatase; postdoctoral investigator; protein protein interaction; protein structure function; spermatogenesis; western blottings

DESCRIPTION (provided by applicant): The work presented in this grant is focused on the long term goal of better understanding the roles of HSF1 and HSF2 in spermatogenesis and early embryogenesis. According to the Center for Disease Control (CDC) infertility affects approximately 6.1 million women and their partners in the United States. Defective expression of heat shock factors leads to several phenotypes including infertility in both males and females. To identify the role(s) of HSF1 and HSF2 in reproduction, these factors must be thoroughly characterized in vivo at various stages of spermatogenesis. The role of HSF2 bookmarking the hsp70i gene in mature spermatozoa must be evaluated. Finally, all gene targets bound by HSF1 and HSF2 must be identified.

描述（由申请人提供）：这项资助的工作集中在更好地理解HSF1和HSF2在精子发生和早期胚胎发生中的作用的长期目标上。根据疾病控制中心（CDC）的数据，在美国，不孕症影响着大约610万妇女及其伴侣。热休克因子的表达缺陷导致几种表型，包括男性和女性的不育。为了确定HSF 1和HSF 2在生殖中的作用，必须在精子发生的各个阶段对这些因子进行体内彻底表征。HSF 2标记hsp70 i基因在成熟精子中的作用必须进行评估。最后，必须鉴定所有与HSF 1和HSF 2结合的基因靶标。