Heat Shock Factor Function in Spermatogenesis

描述(由申请者提供)：这项资助中提出的工作集中于更好地了解HSF1和HSF2在精子发生和早期胚胎发生中的作用的长期目标。根据疾病控制中心(CDC)的数据，在美国，大约有610万妇女及其伴侣受到不孕症的影响。热休克因子的表达缺陷会导致包括男性和女性不育在内的几种表型。为了确定hsf1和hsf2在生殖中的作用(S)，这些因素必须在体内精子发生的不同阶段得到彻底的表征。必须评估HSF2标记HSP70i基因在成熟精子中的作用。最后，必须确定所有与HSF1和HSF2结合的基因靶点。

DESCRIPTION (provided by applicant): The work presented in this grant is focused on the long term goal of better understanding the roles of HSF1 and HSF2 in spermatogenesis and early embryogenesis. According to the Center for Disease Control (CDC) infertility affects approximately 6.1 million women and their partners in the United States. Defective expression of heat shock factors leads to several phenotypes including infertility in both males and females. To identify the role(s) of HSF1 and HSF2 in reproduction, these factors must be thoroughly characterized in vivo at various stages of spermatogenesis. The role of HSF2 bookmarking the hsp70i gene in mature spermatozoa must be evaluated. Finally, all gene targets bound by HSF1 and HSF2 must be identified.