Heat Shock Factor Function in Spermatogenesis

描述（由申请人提供）：这项资助的工作主要是为了更好地理解HSF1和HSF2在精子发生和早期胚胎发生中的作用。根据疾病控制中心（CDC）的数据，在美国，不孕症影响了大约610万女性及其伴侣。热休克因子的表达缺陷导致多种表型，包括男性和女性的不育。为了确定HSF1和HSF2在生殖中的作用，这些因素必须在精子发生的各个阶段在体内得到彻底的表征。必须评估HSF2标记hsp70i基因在成熟精子中的作用。最后，必须确定所有与HSF1和HSF2结合的基因靶标。

DESCRIPTION (provided by applicant): The work presented in this grant is focused on the long term goal of better understanding the roles of HSF1 and HSF2 in spermatogenesis and early embryogenesis. According to the Center for Disease Control (CDC) infertility affects approximately 6.1 million women and their partners in the United States. Defective expression of heat shock factors leads to several phenotypes including infertility in both males and females. To identify the role(s) of HSF1 and HSF2 in reproduction, these factors must be thoroughly characterized in vivo at various stages of spermatogenesis. The role of HSF2 bookmarking the hsp70i gene in mature spermatozoa must be evaluated. Finally, all gene targets bound by HSF1 and HSF2 must be identified.