B. subtilis gene expression & replication perturbation

描述（由申请人提供）：我的目标是了解DNA复制过程中基因表达的系统调节。不能及时完成DNA复制可导致细胞死亡或基因组不稳定，通常与癌症有关。在许多生物体中，复制过程中DNA损伤和其他痛苦原因的影响通常通过转录反应得到缓解。最近，在DNA复制受到干扰的枯草芽孢杆菌细胞的功能基因组分析中，发现了一种新的全局转录反应。这些基因中至少有50个被认为是由dna调控的，dna是一种必需的、保存良好的atp酶、转录因子和复制启动器。我计划在枯草芽孢杆菌中确定由DnaA直接调控的基因，研究DnaA作为转录调节剂是如何被激活的，并研究其靶启动子是如何被结构来响应它的。

DESCRIPTION (provided by applicant): My goal is to understand the systematic modulation of gene expression in response to problems during DNA replication. Failure to complete DNA replication in a timely manner can lead to cell death or genome instability, often associated with cancer. In many organisms, the effects of DNA damage and other causes of distress during replication are often mitigated through transcriptional responses. Recently, a novel global transcriptional response was identified in a functional genomic analysis of Bacillus subtilis cells perturbed in DNA replication. At least 50 of these genes are thought to be regulated by DnaA, an essential and well conserved ATPase, transcription factor, and replication initiator. I plan to identify the genes directly regulated by DnaA in B. subtilis, address how DnaA is activated as a transcriptional regulator, and examine how its target promoters are structured to respond to it.