B. subtilis gene expression & replication perturbation

描述（由申请人提供）：我的目标是了解DNA复制过程中基因表达的系统调节。未能及时完成DNA复制可能导致细胞死亡或基因组不稳定，通常与癌症有关。在许多生物体中，DNA损伤的影响和复制过程中的其他痛苦原因通常通过转录反应来减轻。最近，一种新的全球转录反应被确定在一个功能基因组分析的枯草芽孢杆菌细胞在DNA复制扰动。这些基因中至少有50个被认为是由DnaA调节的，DnaA是一种必需的和保守的ATP酶、转录因子和复制起始因子。我计划在B中鉴定直接受DnaA调控的基因。枯草芽孢杆菌，地址如何DnaA被激活作为一个转录调节因子，并检查如何其目标启动子的结构，以响应它。

DESCRIPTION (provided by applicant): My goal is to understand the systematic modulation of gene expression in response to problems during DNA replication. Failure to complete DNA replication in a timely manner can lead to cell death or genome instability, often associated with cancer. In many organisms, the effects of DNA damage and other causes of distress during replication are often mitigated through transcriptional responses. Recently, a novel global transcriptional response was identified in a functional genomic analysis of Bacillus subtilis cells perturbed in DNA replication. At least 50 of these genes are thought to be regulated by DnaA, an essential and well conserved ATPase, transcription factor, and replication initiator. I plan to identify the genes directly regulated by DnaA in B. subtilis, address how DnaA is activated as a transcriptional regulator, and examine how its target promoters are structured to respond to it.