Continuous separation of biotransformation products

生物转化产物的连续分离

• 批准号: 2768188
• 金额: --
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2768188
• 关键词: Continuous; separation; biotransformation; products

Biotechnology is being increasingly used in industry to allow greener more sustainable processing. Benefits may include milder reaction conditions such as lower temperature/pressure, fewer side-products and higher specificity. Subsequent complex mixtures may be formed during this new approach which commands more sophisticated downstream processing (DSP) in order to isolate and purify the target molecules. This project will develop new continuous flow separation technologies and build on existing work on automated measurement and pH control in continuous flow. Lab-scale continuous solid-liquid and liquid-liquid separation equipment developed and then tested in representative biotransformation work-ups. Both solid/liquid extraction technology is of interest aiming to address challenges such as separation of excess fatty acids or alcohols from ester and amide materials, and more broadly, removing undesirable impurities from Croda's final products. The aqueous solution, that typically contains buffers, enzymes, cofactors and by products, can be recycled. The project will look at reuse of the solution to determine the accumulation of materials and define the purge level to enable a continuous steady-state process. This will provide data for large-scale operation to facilitate industrial take up of the technology. Croda are investigating biocatalysis using enzymes as well as fermentation and other biotransformation routes to speciality chemicals. Advancing Croda's separation and purification capabilities is of high interest. Striving for higher sustainability and higher quality is motivating Croda to seek more efficient approaches to purification. These methods can be applied more generally in chemical production, not just biotechnology

生物技术正越来越多地用于工业，以实现更环保、更可持续的加工。益处可以包括更温和的反应条件，例如更低的温度/压力、更少的副产物和更高的特异性。随后的复杂混合物可能会在这种新方法中形成，这需要更复杂的下游处理（DSP），以分离和纯化靶分子。该项目将开发新的连续流分离技术，并在连续流中自动测量和pH控制的现有工作的基础上。开发了实验室规模的连续固液和液液分离设备，然后在代表性的生物转化工作中进行了测试。固/液萃取技术都是感兴趣的，旨在解决诸如从酯和酰胺材料中分离过量脂肪酸或醇等挑战，更广泛地说，从禾大的最终产品中去除不需要的杂质。通常含有缓冲液、酶、辅因子和副产物的水溶液可以再循环。该项目将研究解决方案的重复使用，以确定材料的积累并定义清除级别，以实现连续的稳态过程。这将为大规模运行提供数据，以促进工业采用该技术。禾大正在研究使用酶的生物催化以及发酵和其他特殊化学品的生物转化途径。提高禾大的分离和纯化能力具有很高的意义。追求更高的可持续性和更高的质量是禾大寻求更有效的净化方法的动力。这些方法可以更普遍地应用于化学生产，而不仅仅是生物技术