The Role of Polycystins in the Regulation of Intracellular Calcium and Vascular R

多囊蛋白在细胞内钙和血管 R 调节中的作用

• 批准号: 7144882
• 负责人: QI QIAN
• 金额: $ 7.4万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-30 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7144882
• 关键词: adrenergic receptor; calcium channel; calcium ion; cell line; gene expression; gene induction /repression; gene mutation; muscle cells; phosphorylation; polycystic kidney; protein structure function; protein transport; sarcoplasmic reticulum; small interfering RNA; vascular smooth muscle; vasoconstriction; voltage /patch clamp

DESCRIPTION (provided by applicant): The PI's long-term objective is to understand the pathogenesis of vascular manifestations associated with autosomal dominant polycystic kidney disease (ADPKD) by applying the fundamental concepts and broad technologies of cellular physiology and molecular biology. ADPKD is the most common life-threatening monogenic disease in the world; vascular manifestations, including hypertension and intracranial aneurysms, are its leading cause of death. Through her NIH (K08)-supported research, Dr. Qian has discovered that Pkd2+/- mutant vascular smooth muscle cells (VSMCs) manifest intracellular calcium ([Ca2+]i) dysregulation, including reductions in (1) the store-operated Ca2+ channel (SOC)-mediated Ca2+ entry, (2) the sarcoplasmic reticulum Ca2+ store, and (3) basal [Ca2+]i; and Pkd2+/- arteries develop hyperactive vasoconstriction in response to adrenergic receptor agonist (phenylephrine) stimulation. The current proposal is designed to further define the mechanisms underlying Pkd2 mutation-associated vascular abnormalities. The central hypothesis is that polycystin-2 (PC2, the protein product of the Pkd2 gene) regulates the activity of SOC channels. Pkd2+/- mutation reduces SOC-mediated Ca2+ entry. resulting VSMC [Ca2+]i dysregulation and abnormal adrenergic receptor trafficking, which in turn cause hyperactive vasoconstriction in response to stimulation. Specific Aim 1 examines whether and how PC2 regulates the cell-surface SOC channels in VSMCs. Specific Aim 2 examines whether and how Pkd2+/- mutation leads to an abnormal adrenergic receptor trafficking and hyperactive vasoconstriction. To complete these aims, the PI will employ established as well as new models, methods, and probes including: heterozygous Pkd1 and Pkd2 mutant mice, lymphoblastoid cell line, specific gene expression (PC1 - the protein products of Pkd1 gene, w/t PC2, mutant PC2- the protein product of D511, a disease causing PKD2 mutant gene, and TRPC1 - the protein product of trpc1 gene that is a SOC subunit) and gene silencing using small interfering RNA (siRNA), intracellular Ca2+ imaging, and patch clamp techniques including perforated whole-cell and cell-attached configurations. This proposal introduces novel concepts regarding the role of PC2 in the regulation of basal [Ca2+]i and alpha1-adrenergic receptor trafficking. The methodologies applied are direct, specific, and achievable. The information gained from the proposed study will greatly enhance our understanding in the pathogenesis of vascular complications in ADPKD and provide potential areas for therapeutic targets.

描述（由申请人提供）： PI的长期目标是通过应用细胞生理学和分子生物学的基本概念和广泛技术，了解常染色体显性遗传性多囊肾病（ADPKD）相关血管表现的发病机制。ADPKD是世界上最常见的危及生命的单基因疾病;血管表现，包括高血压和颅内动脉瘤，是其主要死因。 通过NIH（K 08）支持的研究，钱博士发现Pkd 2 +/-突变的血管平滑肌细胞（VSMCs）表现出细胞内钙（[Ca 2 +]i）失调，包括（1）钙库操纵的钙通道（SOC）介导的钙内流，（2）肌浆网钙库和（3）基础[Ca 2 +]i的减少;和Pkd 2 +/-动脉响应于肾上腺素能受体激动剂（苯肾上腺素）刺激而产生过度活跃的血管收缩。 目前的建议旨在进一步确定Pkd 2突变相关的血管异常的机制。核心假设是多囊蛋白-2（PC 2，Pkd 2基因的蛋白产物）调节SOC通道的活性。Pkd 2 +/-突变减少SOC介导的Ca 2+内流。导致VSMC [Ca 2 +]i失调和异常的肾上腺素能受体运输，这反过来又引起响应于刺激的过度活跃的血管收缩。具体目标1检查PC 2是否以及如何调节VSMC中的细胞表面SOC通道。具体目标2检查Pkd 2 +/-突变是否以及如何导致异常肾上腺素能受体运输和血管收缩亢进。 为了实现这些目标，PI将采用已建立的以及新的模型、方法和探针，包括：杂合子Pkd 1和Pkd 2突变小鼠，淋巴母细胞系，特异性基因表达（PC 1-Pkd 1基因的蛋白产物，w/t PC 2，突变体PC 2-D511的蛋白产物，D511是一种引起疾病的PKD 2突变基因，和TRPC 1-trpc 1基因的蛋白产物，其是SOC亚基）和使用小干扰RNA（siRNA）、细胞内Ca 2+成像和包括穿孔的全细胞和细胞附着构型的膜片钳技术的基因沉默。 该提案介绍了新的概念，关于PC 2在基础[Ca 2 +]i和α 1-肾上腺素能受体贩运的调节中的作用。所采用的方法是直接的、具体的和可实现的。本研究所获得的信息将极大地提高我们对ADPKD血管并发症发病机制的理解，并为治疗靶点提供潜在的领域。