Calcium Buffering Mechanisms in Taste Cells

味觉细胞中的钙缓冲机制

• 批准号: 7065593
• 负责人: KATHRYN MEDLER
• 金额: $ 7.74万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7065593
• 关键词: calcium channel; calcium flux; calcium indicator; calcium transporting ATPase; cell cell interaction; cell population study; cell type; gene expression; laboratory mouse; membrane transport proteins; mitochondria; polymerase chain reaction; sensory feedback; taste; taste buds

DESCRIPTION (provided by applicant): Calcium is an important second messenger for many cellular processes; therefore, its presence is tightly regulated within cells. Cells have developed at least five calcium buffering mechanisms: the plasma membrane Ca2+-ATPase and Na+/Ca2+ exchanger, the Ca2+-ATPase on the endoplasmic reticulum, the mitochondria, and cytosolic calcium buffering proteins. Since a rise in calcium is the final step in taste transduction, the presence of calcium must be tightly regulated in taste cells as well; however, the calcium buffering mechanisms used by taste cells are not known. It is likely that different calcium buffering mechanisms contribute to the formation and modulation of the calcium signal in response to taste stimuli. This is probably critical in determining how a taste cell communicates to other cells. This application intends to characterize the calcium buffering mechanisms that are present in taste cells. It will correlate their expression with the different cell types that are present in taste buds. It has been shown previously that there are two distinct electrophysiological profiles in taste cells, those with voltage gated calcium channels and those without. These two different profiles correlate loosely with cell type. The first hypothesis is that there will be different calcium buffering mechanisms, depending on whether the cells express calcium channels or not. The second hypothesis is that calcium buffering mechanisms are important components in the formation of the cell's response to taste stimuli. The specific aims of this application are: (1) Characterize the expression of calcium buffering mechanisms present in taste cells and correlate their expression to different cell types; (2) Determine the physiological role of calcium buffering mechanisms in taste cells and determine their role in shaping the Ca2+ response to taste stimuli. These aims will be accomplished by using molecular techniques for aim 1 and calcium imaging for aim 2. Results from the proposed experiments will provide new information about how taste cells generate Ca2+ signals and communicate to other cells/afferent neurons. These data will determine if there are differences between taste cells that have calcium influx through voltage gated calcium channels and cells that use the PLC signaling pathway that results in calcium release from intracellular stores. This data will further our understanding about how taste cells generate a signal in response to a taste stimuli.

描述（由申请人提供）：钙是许多细胞过程的重要第二信使;因此，其存在在细胞内受到严格调控。细胞至少有五种钙缓冲机制：质膜Ca ~（2+）-ATP酶和Na ~+/Ca ~（2+）交换器，内质网上的Ca ~（2+）-ATP酶，线粒体和胞质钙缓冲蛋白。由于钙的升高是味觉传导的最后一步，因此在味觉细胞中钙的存在也必须受到严格的调节;然而，味觉细胞使用的钙缓冲机制尚不清楚。很可能不同的钙缓冲机制有助于响应于味觉刺激的钙信号的形成和调节。这可能是决定味觉细胞如何与其他细胞沟通的关键。本申请旨在表征味觉细胞中存在的钙缓冲机制。它将它们的表达与味蕾中存在的不同细胞类型相关联。以前已经表明，在味觉细胞中存在两种不同的电生理特征，具有电压门控钙通道的味觉细胞和不具有电压门控钙通道的味觉细胞。这两种不同的配置文件与细胞类型松散相关。第一个假设是，将有不同的钙缓冲机制，这取决于细胞是否表达钙通道。第二个假设是，钙缓冲机制是形成细胞对味觉刺激的反应的重要组成部分。本申请的具体目的是：（1）表征味觉细胞中存在的钙缓冲机制的表达，并将其表达与不同的细胞类型相关联;（2）确定味觉细胞中钙缓冲机制的生理作用，并确定其在形成对味觉刺激的Ca 2+响应中的作用。这些目标将通过使用分子技术为目标1和钙成像为目标2来实现。实验结果将为味觉细胞如何产生Ca 2+信号并与其他细胞/传入神经元进行通信提供新的信息。这些数据将确定具有通过电压门控钙通道的钙流入的味觉细胞与使用导致钙从细胞内储存释放的PLC信号传导途径的细胞之间是否存在差异。这些数据将进一步加深我们对味觉细胞如何响应味觉刺激产生信号的理解。

项目成果

Sodium/calcium exchangers selectively regulate calcium signaling in mouse taste receptor cells.

• DOI: 10.1152/jn.00118.2010
• 发表时间: 2010-05
• 期刊: Journal of neurophysiology
• 影响因子: 2.5
• 作者: Steven A. Szebenyi;Agnieszka I. Laskowski;K. Medler

Calcium signaling in taste cells: regulation required.

• DOI: 10.1093/chemse/bjq082
• 发表时间: 2010-11
• 期刊: Chemical senses
• 影响因子: 3.5
• 作者: K. Medler

Expression of GABAergic receptors in mouse taste receptor cells.

• DOI: 10.1371/journal.pone.0013639
• 发表时间: 2010-10-26
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Starostik MR;Rebello MR;Cotter KA;Kulik A;Medler KF
• 通讯作者: Medler KF

Ryanodine receptors selectively contribute to the formation of taste-evoked calcium signals in mouse taste cells.

• DOI: 10.1111/j.1460-9568.2010.07463.x
• 发表时间: 2010-12
• 期刊: The European journal of neuroscience
• 作者: Rebello MR;Medler KF
• 通讯作者: Medler KF

Expression of calcium binding proteins in mouse type II taste cells.

小鼠 II 型味觉细胞中钙结合蛋白的表达。

• DOI: 10.1369/0022155411402352
• 发表时间: 2011
• 期刊: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
• 作者: Rebello,MichelleR;Aktas,Adem;Medler,KathrynF
• 通讯作者: Medler,KathrynF