Expression of GABAergic receptors in mouse taste receptor cells.
Expression of GABAergic receptors in mouse taste receptor cells.
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DOI：
10.1371/journal.pone.0013639
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发表时间：
2010-10-26
期刊：
影响因子：
3.7
通讯作者：
Medler KF
中科院分区：
文献类型：
--
作者：
Starostik MR;Rebello MR;Cotter KA;Kulik A;Medler KF
在哺乳动物的味觉传导中已发现多种兴奋性神经递质，对抑制性神经递质的研究较少。由于γ-氨基丁酸(GABA)合成酶谷氨酸脱羧酶(GAD)在小鼠味觉细胞亚群中表达，我们推测GABA信号通路的其他成分可能在这个系统中表达。GABA信号由亲离子受体(GABAA和GABAC)或亲代谢性受体(GABAB)启动，而通过转运体(GATs)重新摄取GABA而终止。利用逆转录聚合酶链式反应(RT-PCR)技术，研究了不同GABA信号分子在小鼠味觉系统中的表达。环状乳突中的味觉受体细胞表达多个GABAA和GABAB受体亚基以及多个GATs。免疫细胞化学分析检测了GABA机械在环状乳头中的分布。外周味觉感受器细胞呈GABAA和GABAB免疫反应阳性。我们还使用了在II型味觉细胞(可以对苦味、甜味或鲜味刺激做出反应)或在III型GAD67表达味觉细胞中表达绿色荧光蛋白(GFP)的转基因小鼠。因此，我们能够确定GABA能受体在一些II型和III型味觉细胞中表达。小鼠的GAT4标记集中在味蕾周围的细胞中，在味蕾的边缘有少量阳性标记的TRCs。定位于II型和III型味觉细胞上的GABA能受体的存在表明，GABA可能调节小鼠味蕾中诱发的味觉反应。
Multiple excitatory neurotransmitters have been identified in the mammalian taste transduction, with few studies focused on inhibitory neurotransmitters. Since the synthetic enzyme glutamate decarboxylase (GAD) for gamma-aminobutyric acid (GABA) is expressed in a subset of mouse taste cells, we hypothesized that other components of the GABA signaling pathway are likely expressed in this system. GABA signaling is initiated by the activation of either ionotropic receptors (GABAA and GABAC) or metabotropic receptors (GABAB) while it is terminated by the re-uptake of GABA through transporters (GATs). Using reverse transcriptase-PCR (RT-PCR) analysis, we investigated the expression of different GABA signaling molecules in the mouse taste system. Taste receptor cells (TRCs) in the circumvallate papillae express multiple subunits of the GABAA and GABAB receptors as well as multiple GATs. Immunocytochemical analyses examined the distribution of the GABA machinery in the circumvallate papillae. Both GABAA-and GABAB- immunoreactivity were detected in the peripheral taste receptor cells. We also used transgenic mice that express green fluorescent protein (GFP) in either the Type II taste cells, which can respond to bitter, sweet or umami taste stimuli, or in the Type III GAD67 expressing taste cells. Thus, we were able to identify that GABAergic receptors are expressed in some Type II and Type III taste cells. Mouse GAT4 labeling was concentrated in the cells surrounding the taste buds with a few positively labeled TRCs at the margins of the taste buds. The presence of GABAergic receptors localized on Type II and Type III taste cells suggests that GABA is likely modulating evoked taste responses in the mouse taste bud.
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影响因子：
2.5
作者：
Clapp, TR;Yang, RB;Kinnamon, JC
通讯作者：
Kinnamon, JC
影响因子：
56.9
作者：
GUASTELLA, J;NELSON, N;KANNER, BI
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KANNER, BI
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Guo, Chenying;Stella, Salvatore L., Jr.;Hirano, Arlene A.;Brecha, Nicholas C.
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Brecha, Nicholas C.
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TOBIN, AJ
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