BIOSENSOR

生物传感器

• 批准号: 7313480
• 负责人: Keith Burridge
• 金额: $ 6.44万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-10 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7313480
• 关键词: bioimaging /biomedical imaging; biological signal transduction; biomedical facility; biosensor device; cell migration; cooperative study; fluorescence resonance energy transfer; fluorescent dye /probe; guanine nucleotide binding protein; high throughput technology; imaging /visualization /scanning; intermolecular interaction; molecular polarity; phosphorylation; reporter genes; technology /technique development; time resolved data

The primary goals for the Biosensor Initiative are to continue to develop biosensors for key regulatory and effector nodes in cell migration and to use existing and proposed biosensors to acquire quantitative data that can be used to develop an experimentally verified theoretical model of cell migration by the Modeling Initiative and others in the modeling community. The Initiative builds on the sensor technologies, fluorescence assays, high throughput microscopy and collaborations from the first CMC funding period to move into Phase II, wherein we plan to visualize signaling events in living cells and to acquire and analyze large microscopic data sets. The modeling will be carried out as a close collaboration with the Modeling Initiative. A major focus of the Initiative will be Rho family regulators since they control key migration nodes. In addition the Initiative will focus on construction of biosensors and photoactivatable reagents, using both existing and new technologies, for key upstream and downstream members of these signaling pathways. In the end we produce a detailed experimental and mathematical analysis of a specific, critical signaling pathway in cell migration.

生物传感器计划的主要目标是继续开发用于细胞迁移关键调控和效应节点的生物传感器，并使用现有和拟议的生物传感器获取可用于研究的定量数据