DISCOVERY

发现

• 批准号: 7313396
• 负责人: Denise J. Montell
• 金额: $ 13.7万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-10 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7313396
• 关键词: Drosophilidae; RNA interference; biomedical facility; cell adhesion; cell biology; cell migration; cell morphology; cell motility; cooperative study; gene expression; helminth genetics; high throughput technology; protein localization

The overall goal of this Initiative is to expand the current Migration Knowledgebase through the identification of cellular genes that regulate cell shape, adhesion and motility using high throughput RNA interference screens in cell- or organism-based assays. In addition, large scale expression screens will be employed in the fruit fly to identify candidate genes that display patterns of expression and intracellular localization consistent with a role in migration. Through coordinated cross-species analyses we will identify a set of genes that play a central and conserved role in cell adhesion and migration.

该倡议的总体目标是扩大现有的移徙知识库 通过使用高通量鉴定调节细胞形状、粘附和运动性的细胞基因， 在基于细胞或生物体的测定中进行RNA干扰筛选。此外，大型表情屏幕将在 在果蝇中用于鉴定显示表达模式和细胞内表达模式的候选基因。 本地化与迁移中的角色一致。通过协调的跨物种分析，我们将确定 一组在细胞粘附和迁移中发挥核心和保守作用的基因。