In vitro models of Neisseria gonorrhoeae infection to study resistance acquisition and inform the selection of novel treatment strategies.

淋病奈瑟菌感染的体外模型，用于研究耐药性获得并为新治疗策略的选择提供信息。

• 批准号: 2809907
• 金额: --
• 依托单位: Liverpool School of Tropical Medicine
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2809907
• 关键词: vitro; models; Neisseria; gonorrhoeae; infection

Antibiotic resistant Neisseria gonorrhoeae is a developing and serious threat. N. gonorrhoeae is currently listed on the WHO priority list of pathogens for which new antibiotics are urgently needed. A major obstacle to the development of new drugs to address the threat of MDR and XDR is the absence of robust and predictable models. This project will aim to develop more representative intracellular and extracellular models of N. gonorrhoeae infection. Kinetic live-cell confocal imaging and flow cytometric methods will be used to characterise infection dynamics in appropriate human cell lines. The quantitative data generated will be used to translate dosing regimens to humans following PK/PD modelling. Developing novel and robust drug development models to investigate the threat of MDR and XDR is essential for the prioritisation of drugs, the selection of drug combinations and regimen selection.

耐抗生素淋病奈瑟菌是一种正在发展的严重威胁。N.淋病目前被列入世卫组织病原体优先清单，迫切需要新的抗生素。开发新药物以解决MDR和XDR威胁的主要障碍是缺乏稳健和可预测的模型。本项目旨在建立更具代表性的细胞内和细胞外模型。淋病感染动态活细胞共聚焦成像和流式细胞术方法将用于在适当的人类细胞系中检测感染动力学。生成的定量数据将用于在PK/PD建模后将给药方案转化为人类。开发新的和强大的药物开发模型来研究MDR和XDR的威胁对于药物的优先级，药物组合和方案选择的选择至关重要。