Pathogenesis of wpk-induced Renal and Cerebral Disease

wpk诱发的肾脑疾病的发病机制

• 批准号: 7033715
• 负责人: VINCENT H GATTONE
• 金额: $ 10.47万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7033715
• 关键词: disease /disorder model; gene interaction; genetic mapping; genetic markers; hydrocephalus; laboratory rat; microarray technology; nucleic acid sequence; pathologic process; polycystic kidney

DESCRIPTION (provided by applicant): Inherited renal cystic diseases, including the various forms of polycystic kidney disease (PKD) are prevalent conditions that usually affect multiple organs. There are numerous human genes, which when mutated, lead to a variety of cystic phenotypes with variable extrarenal manifestations. There are several rodent models, some with mutations in known human PKD genes. Others models represent rodent PKD genes, but could also function as modifier genes for other rodent models and human PKD. However, all of these models have made important contributions to our knowledge of PKD. The present proposal will isolate the rat wpk gene which causes renal changes similar to human autosomal recessive PKD. Additionally, affected rats have a cerebral defect (hydrocephalus with agenesis or hypoplasia of the corpus callosum) similar to that seen in human oro-facial-digital, genitopatellar and cerebro-renal-digital syndromes. Currently we localized the wpk gene to an 8cM region of rat Chromosme 5, a location known to harbor a rodent PKD modifier locus. The long term goal of our research is to identify genes and pathways involved in renal cystogenesis in order to develop therapeutic interventions. We hypothesize that the wpk gene represents a human PKD gene and/or a modifier locus. Our Specific Aim is to: 1) Identify, clone and characterize the wpk gene by crossing the Wistar-wpk rat with inbred Brown Norway rats and using chromosomal markers to localize the gene. Aside from the positional approach, we will identify candidate genes from with the 8cM regions to test and screening rat ESTs from that region. Microarrays will be evaluated for misexpressed mRNAs derived from genes within this 8cM target region. The identification of the wpk gene and its protein product will allow insight into cystogenesis as well as important information on shared pathways in kidney and brain development. This model and the wpk gene are important for 2 major reasons: a) they have cystic disease and unique cerebral pathology similar to a few human conditions and b) the wpk lies in a chromosomal region known to modify other rodent forms of PKD and may be an important modifier locus for PKD (rodent and human).

描述（由申请人提供）：遗传性肾脏囊性疾病，包括各种形式的多囊肾病（PKD）是通常影响多个器官的流行疾病。有许多人类基因，当突变时，导致各种囊性表型与可变的肾外表现。有几种啮齿动物模型，其中一些在已知的人类PKD基因中具有突变。其他模型代表啮齿动物PKD基因，但也可以作为其他啮齿动物模型和人类PKD的修饰基因。然而，所有这些模型都为我们对PKD的认识做出了重要贡献。目前的建议将分离大鼠wpk基因，导致肾脏的变化类似于人类常染色体隐性PKD。此外，受影响的大鼠具有脑缺陷（脑积水伴胼胝体发育不全或发育不全），类似于在人类口面指、生殖髌和肾-指综合征中观察到的缺陷。目前，我们将wpk基因定位于大鼠染色体5的8 cM区域，该位置已知具有啮齿动物PKD修饰基因座。我们研究的长期目标是确定参与肾囊肿形成的基因和途径，以开发治疗干预措施。我们假设wpk基因代表人PKD基因和/或修饰基因座。我们的具体目标是：1）通过将Wistar-wpk大鼠与近交系Brown Norway大鼠杂交并使用染色体标记定位wpk基因来鉴定、克隆和表征wpk基因。除了定位的方法，我们将确定候选基因与8 cM区域测试和筛选大鼠EST从该地区。将评价微阵列中源自该8 cM靶区域内基因的错误表达mRNA。wpk基因及其蛋白产物的鉴定将使我们能够深入了解囊肿发生以及肾脏和大脑发育中共同途径的重要信息。该模型和wpk基因是重要的，主要有两个原因：a）它们具有类似于少数人类病症的囊性疾病和独特的大脑病理学，和B）wpk位于已知修饰其他啮齿类形式的PKD的染色体区域，并且可能是PKD（啮齿类和人类）的重要修饰基因座。

项目成果

Acceleration of the meckel syndrome by near-infrared light therapy.

近红外光疗法加速梅克尔综合征。

• DOI: 10.1159/000332046
• 发表时间: 2011
• 期刊: Nephron extra
• 作者: Lim,Jinhwan;Gattone2nd,VincentH;Sinders,Rachel;Miller,CarolineA;Liang,Yun;Harris,Peter;Watkins3rd,JohnB;Henshel,DianeS
• 通讯作者: Henshel,DianeS

The biomarker enriched proteome of autosomal dominant polycystic kidney disease cyst fluid.

生物标志物富集常染色体显性多囊肾病囊液的蛋白质组。

• DOI: 10.1002/prca.200800163
• 发表时间: 2009
• 期刊: Proteomics. Clinical applications
• 作者: Mason,StephenB;Lai,Xianyin;Bacallao,RobertL;Blazer-Yost,BonnieL;Gattone,VincentH;Wang,KevinC;Witzmann,FrankA
• 通讯作者: Witzmann,FrankA