High Pressure Freezing and Processing Unit

高压冷冻加工机组

• 批准号: 6876422
• 负责人: VINCENT H GATTONE
• 金额: $ 16.34万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-15 至 2006-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876422
• 关键词: biomedical equipment purchase; cryopreservation; electron microscopy; histopathology; immunocytochemistry; tissue /cell preparation; tissue /organ preservation

DESCRIPTION (provided by applicant): The Electron Microscopy Center at the Indiana University School of Medicine has an urgent need for a LEICA EM PACT High Pressure Freezing (HPF) and Processing System. The HPF rapidly freezes microbiopsies, tissue pieces or cultured cells at a rate of >13,000 degrees/second. The automated freeze substitution processor allows these specimens to be uniformly processed. This system represents the newest approach to processing tissues for electron microscopy (EM) without chemical fixation. The EM Center is a multi-user facility dedicated to research and a Research Core Laboratory for the Indiana University School of Medicine. The EM Center has served the research needs of IU investigators since 1961 with strong School and Departmental support for its research contributions. The EM Center has a full-time faculty Director, Dr. Gattone, a full-time manager, Ms Miller, and is available to all IU School of Medicine investigators. The present application is for the Leica High Pressure Freezer and Processing System because of a need by eight, NIH funded investigators. Most investigators need this system to perform immunocytochemistry for fixation sensitive antigens or to avoid potential artifacts due to chemical fixation. However, some projects have very innovative needs, such as Dr. Molitoris' use of the Leica needle microbiopsy system, for samples taken from a rat kidney while viewing it, in vivo, with a two photon confocal microscope. Dr. Evan needs to freeze samples obtained directly from the operating room (OR). The mobility of the Leica HPF allows the unit to be easily transported to the pathology room by the OR or two-photon confocal microscope suite. Two highly qualified EM Specialist, Ms. Miller and Pennington, have expertise in the use and maintenance of this Leica freezing and processing system. The research and physical environment, EM Center personnel and IU School of Medicine institutional support make the IU EM Center an excellent place for the appropriate use of the Leica HPF and Processor System.

描述（由申请人提供）： 印第安纳州大学医学院的电子显微镜中心迫切需要LEICA EM PACT高压冷冻（HPF）和处理系统。HPF以> 13，000度/秒的速度快速冷冻活检组织、组织块或培养细胞。自动冷冻替代处理器可以对这些标本进行统一处理。该系统代表了最新的方法来处理组织的电子显微镜（EM）没有化学固定。EM中心是一个多用户设施，致力于研究和研究核心实验室的印第安纳州大学医学院。EM中心自1961年以来一直服务于IU调查人员的研究需求，并为其研究贡献提供了强大的学校和部门支持。EM中心有一个全职的教师主任，博士加顿，一个全职的经理，女士米勒，并提供给所有医学院的研究人员。本申请用于Leica高压冷冻机和处理系统，因为八名NIH资助的研究人员需要。大多数研究者需要该系统进行免疫细胞化学固定敏感抗原或避免由于化学固定的潜在伪影。然而，一些项目有非常创新的需求，例如Molitoris博士使用Leica针式显微活检系统，在体内用双光子共聚焦显微镜观察大鼠肾脏样本。埃文博士需要冷冻直接从手术室（OR）获得的样本。Leica HPF的移动性使其能够通过手术室或双光子共聚焦显微镜套件轻松地运送到病理室。两位高素质的EM专家，米勒女士和彭宁顿，在使用和维护徕卡冷冻和处理系统方面具有专业知识。研究和物理环境、EM中心人员和IU医学院的机构支持使IU EM中心成为适当使用Leica HPF和处理器系统的绝佳场所。