Bandwidth-Selective, APD-Based Flow Cytometer

基于 APD 的带宽选择性流式细胞仪

• 批准号: 6932100
• 负责人: JAMES F CHRISTIAN
• 金额: $ 44.55万
• 依托单位: RADIATION MONITORING DEVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6932100
• 关键词: computer program /software; computer system design /evaluation; computer system hardware; flow cytometry; lasers; optics; photonics; technology /technique development

DESCRIPTION (provided by applicant): An ultra-compact, high-performance, multi-laser, many-parameter, bandwidth-selective flow cytometer is the expected result of the research and development proposed here. The cellular analysis typically carried out by a device of this type, through the use of lasers and photon detectors, will be moved to a whole new level of capability with the research detailed in this proposal. Specifically, revolutionary developments in the basic components of the cytometer: the focusing optics, the laser-interrogation system, and the photon-detection systems are proposed based upon several exciting advancements in these critical areas. Proposed research in optics will develop a sophisticated laser-focusing aperture that can be used with new powerful and compact solid-state lasers in order to increase the number of lasers used in a many-parameter, flow-cytometer system. State-of-the-art research in miniature, solid-state photon detectors will result in highly sensitive, silicon photodetectors, or avalanche photodiodes, that can be adapted for use in flow cytometers. These photon detectors will be used to replace conventional, cumbersome photomultiplier tubes. The current widespread use of flow cytometers as a method of diagnosing disease, especially cancer, through cell population analysis will benefit highly from the increase in sensitivity and capability provided by development of the high-performance device proposed here. Commercially, flow cytometers have wide market acceptance for use as core diagnostic devices in pathology and immunology departments in many hospitals across the United States. Since the market is established for these devices, our high-performance, extremely compact device should find broad acceptance and provide immediate technological improvement in the diagnosis and science carried out with flow cytometers.

描述（由申请人提供）：超紧凑、高性能、多激光、多参数、带宽选择性流式细胞仪是本文提出的研究和开发的预期结果。通过使用激光器和光子探测器，通常由这种类型的设备进行的细胞分析将通过本提案中详细介绍的研究达到一个全新的能力水平。 具体而言，革命性的发展，在细胞仪的基本组成部分：聚焦光学，激光询问系统，和光子检测系统的基础上提出了几个令人兴奋的进步，在这些关键领域。拟议的光学研究将开发一种复杂的激光聚焦孔径，可与新的强大和紧凑的固态激光器一起使用，以增加多参数流式细胞仪系统中使用的激光器数量。微型固态光子探测器的最新研究将产生高灵敏度的硅光电探测器或雪崩光电二极管，可用于流式细胞仪。这些光子探测器将被用来取代传统的、笨重的光电倍增管。 目前广泛使用的流式细胞仪作为诊断疾病，特别是癌症的方法，通过细胞群分析将大大受益于灵敏度和能力的提高，提供了发展的高性能设备在这里提出。在商业上，流式细胞仪在美国许多医院的病理学和免疫学部门中用作核心诊断设备具有广泛的市场接受度。由于这些设备的市场已经建立，我们的高性能，非常紧凑的设备应该得到广泛的接受，并提供即时的技术改进，在诊断和科学进行流式细胞仪。