Lift-off polymer array approach to axon guidance

轴突引导的剥离聚合物阵列方法

• 批准号: 7169241
• 负责人: David M. Lin
• 金额: $ 37.49万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7169241
• 关键词: Adherence; Afferent Neurons; Anterior; Biological; Biological Assay; Biological Models; Biomedical Engineering; Cells; Chromosome Pairing; Complex; Complex Mixtures; Coupled; Couples; Cues; DNA; Data; Deposition; Detection; Development; Discrimination; Dorsal; Epithelium; Exhibits; Failure; Gene Expression; Genes; Glass; Health behavior; Left; Lifting; Location; Maps; Methodology; Methods; Molecular; Molecular Biology; Mus; Nervous system structure; Neurobiology; Neurons; Noise; Pattern; Physics; Polymers; Positioning Attribute; Printing; Process; RNA; Relative (related person); Research Personnel; Research Project Grants; Sampling; Scanning; Signal Transduction; Slide; Spottings; Standards of Weights and Measures; Surface; Synapses; System; Techniques; Transcript; axon guidance; base; data acquisition; design; improved; multidisciplinary; novel; olfactory bulb; parylene C; programs; research study; response; tool; two-dimensional; vapor

DESCRIPTION (provided by applicant): In the mouse olfactory system, 10 million olfactory sensory neurons (OSNs) extend from the epithelium into the olfactory bulb. Within the bulb, they will target and synapse with just one of ~1800 glomerular targets. This targeting of OSNs to glomeruli represents an intriguing model system in which to study pathfinding and target recognition. What are the molecular mechanisms used by OSNs to identify their appropriate glomerulus in the bulb? In this application to the Bioengineering Research Grant program, a multidisciplinary, collaborative effort that spans neurobiology and physics is proposed that will employ new techniques to identify guidance and targeting cues used by OSNs. We hypothesize that OSNs which target different glomeruli must express different guidance cues that enable them to distinguish among various targets. To identify these cues, we have focused on developing highly sensitive molecular tools. First, we have developed a technique capable of amplifying small amounts of RNA. Second, we have developed a novel microarray printing method that greatly improves the sensitivity of the microarray technique. The combination of a highly sensitive amplification technique coupled with a dramatic improvement in microarray data acquisition will provide us with a powerful screen to identify guidance cues in the olfactory system. In this application, we will further characterize and refine the fabrication of these polymer-based arrays. We will compare them against conventional arrays, and then use them to search for axon guidance cues expressed by OSNs.

描述（由申请人提供）：在小鼠嗅觉系统中，1000万个嗅觉感觉神经元（OSNs）从上皮延伸到嗅球。在球内，它们只会与约1800个肾小球靶点中的一个发生突触。这种针对肾小球的OSNs代表了一个有趣的模型系统，用于研究寻路和目标识别。肾小球的分子机制是什么？在这项生物工程研究资助计划的申请中，提出了一项跨神经生物学和物理学的多学科合作努力，将采用新技术来识别osn使用的引导和靶向线索。我们假设针对不同肾小球的nos必须表达不同的引导信号，使它们能够区分不同的靶标。为了识别这些线索，我们专注于开发高度敏感的分子工具。首先，我们开发了一种能够扩增少量RNA的技术。其次，我们开发了一种新的微阵列打印方法，大大提高了微阵列技术的灵敏度。高灵敏度放大技术与微阵列数据采集技术的显著改进相结合，将为我们提供一个强大的屏幕来识别嗅觉系统中的引导线索。在本应用中，我们将进一步表征和改进这些聚合物基阵列的制造。我们将它们与传统阵列进行比较，然后使用它们来搜索由osn表达的轴突引导线索。