The genetics of flowering in raspberry and blackberry - what makes a primocane?

本博士项目的目的是了解两种覆盆子类型中原果与花果的结果习性和早熟性的遗传控制，并研究这些性状在黑莓中的对应关系。为了绘制控制这些性状的qtl，学生将利用现有的育种种群，在东马岭红毛茛育种联盟。在整个育种计划中，将对结果习性进行详细的表征，并使用SNP标记对最合适的家族进行基因分型以进行定位。在此过程中，该项目将有助于开发目前在基因组资源可用性方面落后于草莓的红莓分子工具。本博士项目的目的是了解两种覆盆子类型中原果与花果的结果习性和早熟性的遗传控制，并研究这些性状在黑莓中的对应关系。为了绘制控制这些性状的qtl，学生将利用现有的育种种群，在东马岭红毛茛育种联盟。在整个育种计划中，将对结果习性进行详细的表征，并使用SNP标记对最合适的家族进行基因分型以进行定位。在此过程中，该项目将有助于开发目前在基因组资源可用性方面落后于草莓的红莓分子工具。基于QTL定位数据，开发遗传标记，针对不同的结果习性进行标记辅助育种。为了更详细地了解开花的控制，我们将在开花习性不同的品种中研究所选候选基因的表达。此外，基于我们在遗传转化方面的强大专业知识，将为覆盆子开发一种基因编辑方案，并将使用基因编辑系测试至少两个候选基因的功能，作为概念证明。

The aim of this PhD project is to understand the genetic control of primocane vs. floricane fruiting habit and earliness of fruiting in both raspberry types in the first instance and investigate the correspondence of these traits in blackberry. To map QTLs controlling these traits the student will utilize existing breeding populations available in the East Malling Rubus Breeding Consortium. Detailed characterization of fruiting habits will be carried out throughout the breeding program and the most suitable families will be genotyped for mapping using SNP markers. In doing so, this project will contribute to the development of molecular tools in Rubus which currently lags behind strawberry in availability of genomic resources.The aim of this PhD project is to understand the genetic control of primocane vs. floricane fruiting habit and earliness of fruiting in both raspberry types in the first instance and investigate the correspondence of these traits in blackberry. To map QTLs controlling these traits the student will utilize existing breeding populations available in the East Malling Rubus Breeding Consortium. Detailed characterization of fruiting habits will be carried out throughout the breeding program and the most suitable families will be genotyped for mapping using SNP markers. In doing so, this project will contribute to the development of molecular tools in Rubus which currently lags behind strawberry in availability of genomic resources.Based on QTL mapping data, genetic markers will be developed to facilitate marker assisted breeding targeting different fruiting habits. To understand the control of flowering in more detail, the expression of selected candidate genes will be studied in cultivars with contrasting flowering habits. Moreover, based on our strong expertise in genetic transformation, a gene-editing protocol will be developed for raspberries, and the function of at least two candidate genes will be tested using gene-edited lines as a proof of concept.