Characterizing the Phenotype of Staphylococcal Biofilms

表征葡萄球菌生物膜的表型

• 批准号: 7178539
• 负责人: JULIA M ROSS
• 金额: $ 36.54万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE COUNTY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7178539
• 关键词: Address; Adhesions; Affect; Antibiotic Resistance; Antibiotic Therapy; Antibiotics; Architecture; Area; Bacteria; Bacterial Adhesion; Binding; Biocompatible Materials; Blood Circulation; Blood Platelets; Capsid Proteins; Cardiovascular system; Cell Adhesion Molecules; Cell Wall; Cells; Characteristics; Chronic; Collagen; Condition; Data; Development; Endocarditis; Fibrinogen; Fibronectins; Genus staphylococcus; Growth; Immune system; Infection; Intracellular Membranes; Lead; Leukocytes; Liquid substance; Membrane; Membrane Proteins; Microbe; Microbial Biofilms; Numbers; Pathogenesis; Pattern; Penetration; Phenotype; Population; Prevention; Property; Proteins; Proteomics; Protocols documentation; Research; Research Personnel; Resistance; Resort; Serum; Site; Staging; Staphylococcus aureus; Structure; Surface; Suspension Culture; Suspension substance; Suspensions; Tissues; Vancomycin; Vascular Graft; adhesion receptor; base; design; flasks; novel therapeutics; programs; protein expression; response; shear stress; success; therapeutic target; three dimensional structure

DESCRIPTION (provided by applicant): The broad objective of this research is to comprehensively characterize the structure, growth and maturation of Staphylococcus aureus biofilms as well as the phenotypic similarities and differences between staphylococci grown as biofilm versus planktonic cells. In particular, we will probe the adhesion properties of both mature biofilms and planktonic cells since they are critical to both biofilm initiation and immune system response. Elucidation of these phenotypic differences may ultimately lead to the design of novel therapeutic strategies for staphylococcal biofilm prevention and control. We hypothesize that the repertoire and number of surface expressed S. aureus proteins vary depending on the mode of growth. We further hypothesize that this variance will directly affect the adhesion properties of both the biofilm and detached planktonic bacteria under shear conditions. The specific aims of the project are to (1) investigate the architecture of staphylococcal biofilms grown on protein surfaces in the presence and absence of serum under controlled shear conditions, including growth and developmental characteristics, (2) characterize adhesion properties of staphylococcal biofilms and detached planktonic cells grown in the presence and absence of serum as a function of wall shear stress and (3) characterize the intracellular, membrane-bound and secreted protein expression patterns as a function of how staphylococci are grown. Suspension cultures at various growth stages, surface-attached cells in various stages of biofilm development, and planktonic populations shed from biofilms will be evaluated and compared. Completion of these specific aims will provide a rational basis for the design of new therapeutic molecules.

描述（由申请人提供）： 这项研究的主要目标是全面表征金黄色葡萄球菌生物膜的结构、生长和成熟，以及生物膜生长的葡萄球菌与浮游细胞之间表型的相似性和差异。特别是，我们将探讨成熟生物膜和浮游细胞的粘附特性，因为它们对于生物膜启动和免疫系统反应都至关重要。阐明这些表型差异可能最终导致设计预防和控制葡萄球菌生物膜的新治疗策略。我们假设表面表达的金黄色葡萄球菌蛋白的库和数量根据生长模式而变化。我们进一步假设这种差异将直接影响生物膜和分离的浮游细菌在剪切条件下的粘附特性。该项目的具体目标是（1）研究在受控剪切条件下存在和不存在血清的情况下在蛋白质表面上生长的葡萄球菌生物膜的结构，包括生长和发育特征，（2）表征在存在和不存在血清的情况下生长的葡萄球菌生物膜和分离的浮游细胞的粘附特性作为壁剪切应力的函数，以及（3）表征 细胞内、膜结合和分泌蛋白表达模式作为葡萄球菌生长方式的函数。将评估和比较不同生长阶段的悬浮培养物、生物膜发育不同阶段的表面附着细胞以及从生物膜脱落的浮游种群。这些具体目标的完成将为新治疗分子的设计提供合理的基础。