Reliable Assembly of Cloned DNA Fragments

克隆 DNA 片段的可靠组装

• 批准号: 7108928
• 负责人: John Thomas Mulligan
• 金额: $ 36.62万
• 依托单位: BLUE HERON BIOTECHNOLOGY, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-05 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7108928
• 关键词: DNA; DNA binding protein; DNA methylation; artificial chromosomes; binding sites; biotechnology; expression cloning; high throughput technology; macromolecule; method development; methyltransferase; molecular assembly /self assembly; nucleic acid chemical synthesis; plasmids; restriction endonucleases; transfection /expression vector

DESCRIPTION (provided by applicant): Our goal in this project is to develop a uniform method for assembling large DNA molecules by ligation of sticky-ended fragments, regardless of the sequence, the fragment length, or the presence of restriction enzyme sites. If we are successful, this technology could be used to assemble full-length cDNAs, mammalian genes, knockout constructs, gene clusters, and even whole BACs or bacterial genomes. The combination of a universal fragment assembly technology with Blue Heron's core technology, gene synthesis, will provide researchers with rapid, cost-effective access to any DNA sequence, regardless of size or sequence composition. It will speed the pace of research by allowing scientists to focus on experiments rather than cutting and pasting DNA molecules. It wll also enable new projects that would be impossible or impractically expensive without full control of large DNA sequences.

描述(由申请人提供)：我们在这个项目中的目标是开发一种统一的方法，通过连接粘性末端片段来组装大的DNA分子，而不考虑序列、片段长度或限制酶位点的存在。如果我们成功，这项技术可以用来组装全长cDNA、哺乳动物基因、敲除结构、基因簇，甚至整个BAC或细菌基因组。将通用片段组装技术与蓝鹭的核心技术基因合成相结合，将为研究人员提供快速、经济高效的任何DNA序列，无论其大小或序列组成如何。它将使科学家能够专注于实验，而不是剪切和粘贴DNA分子，从而加快研究的步伐。它还将使新的项目成为可能，如果没有对大型DNA序列的完全控制，这些项目将是不可能的或昂贵的。