Reliable Assembly of Cloned DNA Fragments

克隆 DNA 片段的可靠组装

• 批准号: 7224864
• 负责人: John Thomas Mulligan
• 金额: $ 41.33万
• 依托单位: BLUE HERON BIOTECHNOLOGY, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-05 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7224864
• 关键词: Algorithms; Bacterial Genome; Base Pairing; Binding; Binding Proteins; Binding Sites; Cells; Cloning; Codon Nucleotides; Compatible; Computer software; DNA; DNA Methyltransferase; DNA Modification Methylases; DNA Sequence; Development; Digestion; Enzymes; Gene Cluster; Genes; Goals; Heel; In Vitro; Length; Ligation; Location; Methods; Methylation; Methyltransferase; Molecular; Molecular Biology; Numbers; Paste substance; Phase; Placement; Plasmids; Pliability; Positioning Attribute; Preparation; Principal Investigator; Process; Production; Proteins; Protocols documentation; Range; Reaction; Research; Research Personnel; Resistance; Scientist; Site; Solid; Speed; Standards of Weights and Measures; Technology; Testing; base; cost; design; gene synthesis; in vivo; knockout gene; new technology; plasmid DNA; programs; research study; restriction enzyme; size; vector

DESCRIPTION (provided by applicant): Our goal in this project is to develop a uniform method for assembling large DNA molecules by ligation of sticky-ended fragments, regardless of the sequence, the fragment length, or the presence of restriction enzyme sites. If we are successful, this technology could be used to assemble full-length cDNAs, mammalian genes, knockout constructs, gene clusters, and even whole BACs or bacterial genomes. The combination of a universal fragment assembly technology with Blue Heron's core technology, gene synthesis, will provide researchers with rapid, cost-effective access to any DNA sequence, regardless of size or sequence composition. It will speed the pace of research by allowing scientists to focus on experiments rather than cutting and pasting DNA molecules. It wll also enable new projects that would be impossible or impractically expensive without full control of large DNA sequences.

描述（由申请人提供）：我们在该项目中的目标是开发一种通过连接粘性末端片段组装大DNA分子的统一方法，而不管序列、片段长度或限制性内切酶位点的存在。如果我们成功了，这项技术可以用来组装全长cDNA、哺乳动物基因、敲除构建体、基因簇，甚至整个BAC或细菌基因组。通用片段组装技术与Blue Heron的核心技术基因合成相结合，将为研究人员提供快速，具有成本效益的任何DNA序列，无论大小或序列组成。它将加快研究的步伐，让科学家专注于实验，而不是剪切和粘贴DNA分子。它还将使新的项目，将是不可能的或不切实际的昂贵没有完全控制的大型DNA序列。