A Dual Electrospray/Photoionization Source

双电喷雾/光电离源

• 批准号: 7047713
• 负责人: Jack Albert Syage
• 金额: $ 12.52万
• 依托单位: SYAGEN TECHNOLOGY, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7047713
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; chemical structure; drug screening /evaluation; electrospray ionization mass spectrometry; photochemistry; proteomics

DESCRIPTION (provided by applicant): In phase II we propose an experimental plan that will lead to a commercial quality electrospray/photoionization (ES/PI) source. We plan to: (i) optimize and build a pre-commercial prototype ES/PI source for use on commercial mass spectrometers, and (ii) develop and demonstrate key applications that highlight the unique benefits of the ES/PI source, The goals of the phase I project were met and a prototype ES/PI was built and tested on a Micromass LCT and an Agilent MSD mass spectrometer. Since phase I, Syagen has performed additional tests, and has adapted a new source to a Waters ZQ. The ES/PI performance was measured as a function of flow rate, heating, PI position, and ES ionization (ESI) voltage. Tests were conducted using a variety of compounds not ionized efficiently by ESI (e.g., steroids, phenols, aldehydes, etc.). PI ionized these compounds efficiently. Further tests were performed on drug and protein mixtures to examine the potential to analyze both classes of compounds simultaneously. The significance and importance of this work is high. Developing new ionization sources for life science research is a major thrust in mass spectrometry as witnessed by the awarding of the 2002 Nobel Prize for ESI and laser desorption/ionization (e.g., MALDI) of biological molecules. This proposed work will greatly expand the capabilities of ESI making possible the development of new advanced methods for high-throughput analysis. There is already interest in this technology by commercial MS vendors. If we are successful with the phase II work, we are confident of finding a commercialization partner for the ES/PI source.

描述(由申请人提供)：在第二阶段，我们提出了一项实验计划，该计划将导致商业质量的电喷雾/光电离化(ES/PI)源。我们计划：(I)优化和构建用于商业质谱仪的预商业化原型ES/PI源，以及(Ii)开发和演示突出ES/PI源独特优势的关键应用，实现了第一阶段项目的目标，并建立了ES/PI原型，并在MicroMassLCT和Agilent MSD质谱仪上进行了测试。自第一阶段以来，赛根斯进行了额外的测试，并将一个新的来源改装为Waters ZQ。测量了ES/PI性能随流速、加热、PI位置和ES电离(ESI)电压的变化。测试使用了不被ESI有效电离的各种化合物(例如，类固醇、酚、醛等)。PI有效地电离了这些化合物。对药物和蛋白质混合物进行了进一步的测试，以检查同时分析这两类化合物的可能性。这项工作的意义和重要性很高。为生命科学研究开发新的电离源是质谱学的一个主要推动力，2002年生物分子的ESI和激光解吸/电离(例如，MALDI)诺贝尔奖的颁发证明了这一点。这项拟议的工作将极大地扩展电喷雾化分析的能力，使开发用于高通量分析的新的先进方法成为可能。商业MS供应商已经对这项技术感兴趣。如果我们的第二阶段工作成功，我们有信心为ES/PI源找到一个商业化合作伙伴。