A Dual Electrospray/Photoionization Source

在第二阶段，我们提出了一个实验计划，该计划将导致商业质量 电喷雾/光电离(ES/PI)源。我们计划：(I)优化和构建一个商业预案 用于商用质谱计的ES/PI原型源，以及(Ii)开发和 演示突出ES/PI源的独特优势的关键应用程序、 满足了第一阶段项目，并建立了原型ES/PI，并在微质量LCT和AN上进行了测试 安捷伦MSD质谱仪。自第一阶段以来，Syagen进行了额外的测试，并 改编了一个新的信号源到Waters ZQ。ES/PI性能作为流量的函数进行了测量 速度、加热、PI位置和ES电离(ESI)电压。测试使用了各种不同的 未被ESI有效电离的化合物(例如，类固醇、酚、醛等)。PI将这些电离 高效合成化合物。对药物和蛋白质混合物进行了进一步的测试，以检查 有可能同时分析这两类化合物。的意义和重要性 这项工作要求很高。为生命科学研究开发新的电离源是 2002年诺贝尔ESI和激光奖颁奖仪式见证的质谱学 生物分子的解吸/电离(例如，MALDI)。这项拟议的工作将大大扩展 ESI的能力使开发新的先进方法成为可能 高通量分析。商业MS供应商已经对这项技术感兴趣。如果 我们成功地完成了第二阶段的工作，我们有信心找到一个商业化的合作伙伴 ES/PI源。

In Phase II we propose an experimental plan that will lead to a commercial quality electrospray/photoionization (ES/PI) source. We plan to: (i) optimize and build a pre-commercial prototype ES/PI source for use on commercial mass spectrometers, and (ii) develop and demonstrate key applications that highlight the unique benefits of the ES/PI source, The goals of the Phase I project were met and a prototype ES/PI was built and tested on a Micromass LCT and an Agilent MSD mass spectrometer. Since Phase I, Syagen has performed additional tests, and has adapted a new source to a Waters ZQ. The ES/PI performance was measured as a function of flow rate, heating, PI position, and ES ionization (ESI) voltage. Tests were conducted using a variety of compounds not ionized efficiently by ESI (e.g., steroids, phenols, aldehydes, etc.). PI ionized these compounds efficiently. Further tests were performed on drug and protein mixtures to examine the potential to analyze both classes of compounds simultaneously. The significance and importance of this work is high. Developing new ionization sources for life science research is a major thrust in mass spectrometry as witnessed by the awarding of the 2002 Nobel Prize for ESI and laser desorption/ionization (e.g., MALDI) of biological molecules. This proposed work will greatly expand the capabilities of ESI making possible the development of new advanced methods for high-throughput analysis. There is already interest in this technology by commercial MS vendors. If we are successful with the Phase II work, we are confident of finding a commercialization partner for the ES/PI source.