A Dual Electrospray/Photoionization Source

我们提出了一种将电喷雾(ES)装置集成到大气压光电离源(PI)中的方法。我们将使用一种专利方法来产生带电液滴，这些液滴将受到电荷排斥，形成挥发的分析物，而不产生ES阳离子。正离子光谱将只由PI引起。通过这种方法，PI的好处可以扩展到非挥发性和热不稳定的化合物。PI已被证明是分析各种化合物的通用电离源，在许多情况下，电离(ESI)或常压化学电离(A PCI)无法检测到的化合物。PI特别适合于类药物化合物，并且不容易受到竞争电荷和离子抑制的影响，这会影响ESI和APCI质谱图。这项提案是一项重新提交的提案，其中包含了极大地提高成功概率的新结果。我们相信，如果提供SBIR种子资金来测试第一阶段的可行性，并解决第二阶段的研发问题，ES/PI来源将立即商业化。ES/PI源还可以用作ES电离源，从而为大分子提供双重ESI和PI能力。后一种战略将导致制药业迅速接受和广泛使用。建议的商业应用：制药行业在速度、定量和通用性方面对分子分析技术提出了越来越高的要求。开发双ESI/PI源将极大地扩展能力，并将被广泛接受为增强型ESI源。

We propose a method to integrate an electrospray (ES) device to an atmospheric pressure photoionization (P I) source. We will employ a proprietary method to generate charged droplets that will undergo charge-repulsion to form volatilized analyte without producing ES positive ions. The positive ion spectrum will be due to PI only. By this means, the benefits of PI can be extended to non-volatile and thermally- labile compounds. PI has proven to be a general purpose ionization source for analyzing a wide variety of compounds, in many cases compounds not detectable by ES ionization (ESI) or atmospheric pressure chemical ionization (A PCI). PI is particularly well suited for drug-like compounds, and is much less susceptible to competition-for- charge and ion-suppression that affects ESI and APCI mass spectra. This proposal is a resubmission that contains new results that greatly raise the probability of success. We are confident that if provided SBIR seed money to test the feasibility in Phase I and solve the R&D issues in Phase II, that the ES/PI source would be immediately commercializable. The ES/PI source could also operate as an ES ionization source thereby providing dual ESI and PI capabilities for large molecules. The latter strategy would lead to rapid acceptance and widespread use in the pharmaceutical industry. PROPOSED COMMERCIAL APPLICATION: The pharmaceutical industry places increasingly greater demands on molecular analysis technology in terms of speed, quantitation, and generality. Development of a dual ESI/PI source would greatly expand capabilities and would be widely accepted into use as an enhanced ESI source.