CONFOCAL MICROSCOPY AND IMAGE ANALYSIS CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。该核心为TNPRC的每个部门以及多个机构的众多附属研究科学家提供最先进的共聚焦显微镜，多标签荧光标记和检测以及图像分析支持。Core有一个Leica TCS SP2激光扫描共聚焦显微镜系统，配备3个激光器，6条激光线可用，能够同时收集四个通道（3个荧光通道和一个差分干涉对比度通道）的信息。该系统连接在两台显微镜上，一台是立式显微镜（DMRE），另一台是倒置显微镜（DMIRE2），可以对固定制剂和活细胞进行共聚焦显微镜观察。我们有一个单独的工作站来运行徕卡软件来分析收集到的数据。我们也有Volocity软件，用于在三维空间中呈现数据。透射光的图像分析系统是基于徕卡DMRE显微镜，Spot相机和最新的ImagePro软件在一个专门的工作站，供调查人员在图像分析中使用。共聚焦系统提供了以下好处：a)多维成像，因为可以获得四个维度的图像，长度（x轴），宽度（y轴），深度（z轴）和时间(t)。b)分辨率提高。c)对比改善。e)多色成像。几种荧光染料可以同时成像

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core provides state-of-the-art confocal microscopy, multilabel fluorescent labeling and detection, and image analysis support to every Division in the TNPRC, and to numerous affiliate research scientists at several institutions. The Core has a Leica TCS SP2 laser scanning confocal microscope system equipped with 3 lasers, with 6 laser lines available, capable of simultaneously collecting information in four channels (3 fluorescent and one for differential interference contrast). The system is attached to two microscopes an upright (DMRE) and an inverted (DMIRE2), that allow for confocal microscopy of fixed preparations and also living cells. We have a separate workstation to run the Leica software for the analysis of the data collected. We also have Volocity Software for the rendition of the data in 3 dimensions. The Image analysis system for transmitted light is based in the Leica DMRE microscope, a Spot camera and the latest ImagePro software in a dedicated workstation for investigators use in image analysis. The confocal system offers such benefits as: a) Multi-dimensional imaging, as it is possible to obtain images in four dimensions, length (x axis), width (y axis), depth (z axis) and time (t). b) Resolution improvement. c) Contrast improvement. e) Multicolor imaging. Several fluorochromes can be imaged at the same time