CONFOCAL MICROSCOPY AND IMAGE ANALYSIS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该核心为TNPRC的每个部门以及多个机构的众多附属研究科学家提供最先进的共聚焦显微镜、多标记荧光标记和检测以及图像分析支持。核心拥有徕卡TCS SP2激光扫描共聚焦显微镜系统，配备3个激光器，6条激光线路，能够同时收集四个通道的信息(3个荧光和一个用于差分干涉对比)。该系统连接到两个显微镜，一个是立式的(DMRE)，另一个是倒置的(DMIRE2)，可以对固定的制剂和活细胞进行共焦显微镜观察。我们有一个单独的工作站来运行徕卡软件来分析收集的数据。我们也有Volocity软件，可以在3个维度上再现数据。透射光图像分析系统基于徕卡DMRE显微镜、SPOT相机和最新的ImagePro软件，在专用工作站中供研究人员用于图像分析。共焦系统具有以下优点：a)多维成像，因为可以获得长度(x轴)、宽度(y轴)、深度(z轴)和时间(T)四个维度的图像。B)提高分辨率。C)对比度改善。D)多色成像。可以同时成像多个荧光染料。 去年，五名新使用者接受了自行使用共焦显微镜的培训。此外，核心人员在145个时隙为训练有素的用户提供了580个小时的协助。该核心执行了470项成像服务，包括活体成像和产生超过200 GB数据的2或3色显微镜。核心直接促成了9份新出版物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core provides state-of-the-art confocal microscopy, multilabel fluorescent labeling and detection, and image analysis support to every Division in the TNPRC, and to numerous affiliate research scientists at several institutions. The Core has a Leica TCS SP2 laser scanning confocal microscope system equipped with 3 lasers, with 6 laser lines available, capable of simultaneously collecting information in four channels (3 fluorescent and one for differential interference contrast). The system is attached to two microscopes an upright (DMRE) and an inverted (DMIRE2), that allow for confocal microscopy of fixed preparations and also living cells. We have a separate workstation to run the Leica software for the analysis of the data collected. We also have Volocity Software for the rendition of the data in 3 dimensions. The Image analysis system for transmitted light is based in the Leica DMRE microscope, a Spot camera and the latest ImagePro software in a dedicated workstation for investigators use in image analysis. The confocal system offers such benefits as: a) Multi-dimensional imaging, as it is possible to obtain images in four dimensions, length (x axis), width (y axis), depth (z axis) and time (t). b) Resolution improvement. c) Contrast improvement. d) Multicolor imaging. Several fluorochromes can be imaged at the same time. In the last year five new users were trained to use the confocal microscope by themselves. In addition, core personnel assisted less trained users in 145 timeslots for 580 hours. The core performed 470 imaging services, including in vivo imaging and 2 or 3 color microscopy that generated more than 200 gigabytes of data. The core directly contributed to 9 new publications.