CONFOCAL MICROSCOPY AND IMAGE ANALYSIS CORE

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该核心为TNPRC的每个部门以及几个机构的众多附属研究科学家提供最先进的共聚焦显微镜、多标记荧光标记和检测以及图像分析支持。 Core配备了Leica TCS SP2激光扫描共聚焦显微镜系统，配备3个激光器，有6条激光线可用，能够同时收集四个通道的信息（3个荧光通道和一个微分干涉对比通道）。 该系统连接到两个显微镜;一个直立（DMRE）和一个倒置（DMIRE 2），允许固定制剂和活细胞的共聚焦显微镜。我们有一个单独的工作站来运行Leica软件，以分析收集的数据。我们也有Volocity软件来渲染三维数据。透射光图像分析系统基于Leica DMRE显微镜、Spot摄像头和最新的ImagePro软件，在专用工作站中供研究人员用于图像分析。我们从CRI获得了Nuance多光谱相机和用于图像分析的互补Inform软件。我们正在使用Nuance相机捕获和分析免疫组织化学图像。这款相机的多光谱功能使我们能够比以往更好地分离各个标签的颜色。在过去的一年里，四个新用户接受了培训，可以自己使用共焦显微镜。 此外，核心人员在150个时段内为培训程度较低的用户提供了600个小时的协助。 该核心执行了300项成像服务，包括体内成像和2或3色显微镜，产生了超过400千兆字节的数据。 我们已经培训了3名用户使用Inform软件进行图像分析，但与核心经理一起在不到一个月的时间内生成了500 GB。 这一新设备使用良好，效果良好。该核心直接为13份新出版物提供了资料，其中两份刊登在该期刊的封面上。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This Core provides state-of-the-art confocal microscopy, multilabel fluorescent labeling and detection, and image analysis support to every Division in the TNPRC, and to numerous affiliate research scientists at several institutions. The Core has a Leica TCS SP2 laser scanning confocal microscope system equipped with 3 lasers, with 6 laser lines available, capable of simultaneously collecting information in four channels (3 fluorescent and one for differential interference contrast). The system is attached to two microscopes; an upright (DMRE) and an inverted (DMIRE2), that allow for confocal microscopy of fixed preparations and also living cells. We have a separate workstation to run the Leica software for the analysis of the data collected. We also have Volocity Software for rendering data in 3 dimensions. The Image analysis system for transmitted light is based in the Leica DMRE microscope, a Spot camera and the latest ImagePro software in a dedicated workstation for investigators use in image analysis. We acquired a Nuance multi-spectral camera from CRI and the complementary Inform software for Image analysis. We are using the Nuance camera to capture and analyze images from immunohistochemistry. The multispectral capability of this camera allows us to separate the colors of individual labels better than ever before. In the last year four new users were trained to use the confocal microscope by themselves. In addition, core personnel assisted less trained users in 150 timeslots for 600 hours. The core performed 300 imaging services, including in vivo imaging and 2 or 3 color microscopy that generated more than 400 gigabytes of data. We have trained 3 users in image analysis with the Inform software but together with the core manager generated 500 GB in less than a month. This new piece of equipment is well used and is providing good results. The core directly contributed to 13 new publications two of which were featured on the cover of the journal.