CONFOCAL MICROSCOPY AND IMAGE ANALYSIS CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该核心为TNPRC的每个部门以及多个机构的众多附属研究科学家提供最先进的共聚焦显微镜、多标记荧光标记和检测以及图像分析支持。核心拥有徕卡TCS SP2激光扫描共聚焦显微镜系统，配备3个激光器，6条激光线路，能够同时收集四个通道的信息(3个荧光和一个用于差分干涉对比)。该系统连接到两个显微镜；一个直立的(DMRE)和一个倒置的(DMIRE2)，允许对固定制剂和活细胞进行共焦显微镜观察。我们有一个单独的工作站来运行徕卡软件来分析收集的数据。我们也有用于在3维空间中渲染数据的Volocity软件。透射光图像分析系统基于徕卡DMRE显微镜、SPOT相机和最新的ImagePro软件，在专用工作站中供研究人员用于图像分析。我们从CRI购买了一台Nuance多光谱相机和补充的INFORM图像分析软件。我们正在使用Nuance相机捕捉和分析免疫组织化学的图像。这款相机的多光谱功能使我们能够比以往任何时候都更好地分离单个标签的颜色。去年，四名新使用者接受了自学使用共焦显微镜的培训。此外，核心人员在150个时隙为训练有素的用户提供了600小时的协助。该核心执行了300项成像服务，包括活体成像和产生超过400 GB数据的2或3色显微镜。我们使用INFORM软件培训了3名用户进行图像分析，但与核心管理器一起在不到一个月的时间内生成了500 GB的数据。这台新设备使用得很好，效果很好。核心直接促成了13份新出版物，其中两份登上了该期刊的封面。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This Core provides state-of-the-art confocal microscopy, multilabel fluorescent labeling and detection, and image analysis support to every Division in the TNPRC, and to numerous affiliate research scientists at several institutions. The Core has a Leica TCS SP2 laser scanning confocal microscope system equipped with 3 lasers, with 6 laser lines available, capable of simultaneously collecting information in four channels (3 fluorescent and one for differential interference contrast). The system is attached to two microscopes; an upright (DMRE) and an inverted (DMIRE2), that allow for confocal microscopy of fixed preparations and also living cells. We have a separate workstation to run the Leica software for the analysis of the data collected. We also have Volocity Software for rendering data in 3 dimensions. The Image analysis system for transmitted light is based in the Leica DMRE microscope, a Spot camera and the latest ImagePro software in a dedicated workstation for investigators use in image analysis. We acquired a Nuance multi-spectral camera from CRI and the complementary Inform software for Image analysis. We are using the Nuance camera to capture and analyze images from immunohistochemistry. The multispectral capability of this camera allows us to separate the colors of individual labels better than ever before. In the last year four new users were trained to use the confocal microscope by themselves. In addition, core personnel assisted less trained users in 150 timeslots for 600 hours. The core performed 300 imaging services, including in vivo imaging and 2 or 3 color microscopy that generated more than 400 gigabytes of data. We have trained 3 users in image analysis with the Inform software but together with the core manager generated 500 GB in less than a month. This new piece of equipment is well used and is providing good results. The core directly contributed to 13 new publications two of which were featured on the cover of the journal.