Mechanisms Regulating Contractile Ring in Fission Yeast

裂殖酵母收缩环的调节机制

• 批准号: 7241579
• 负责人: Dawn M CLIFFORD-HART
• 金额: $ 4.88万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7241579
• 关键词: Actomyosin; Aneuploidy; Binding; Biochemical; Biochemistry; Biological Assay; Cell Cycle; Cell Division Process; Cell Nucleus; Cell division; Cells; Chromosomes; Cytokinesis; Enzymes; Eukaryota; Eukaryotic Cell; Event; Exhibits; F-Actin; Family; Fission Yeast; Generations; Genetic; Genetic Materials; Genomics; Goals; Homologous Gene; Human; Knowledge; Lead; Location; Medial; Mediating; Methods; Microscopy; Mitosis; Mitotic; Molecular; Molecular Biology; Myosin Type II; Organism; Phase; Phosphoric Monoester Hydrolases; Physiological; Placement; Play; Positioning Attribute; Process; Protein phosphatase; Proteins; Recruitment Activity; Role; Structure; Substrate Interaction; System; Testing; Work; Yeasts; anillin; base; cancer prevention; carcinogenesis; cell preparation; design; enzyme substrate; improved; in vivo; molecular imaging; novel; prevent; response; transmission process

DESCRIPTION (provided by applicant): Equal division of the physical cell directly influences the transfer of a complete set of chromosomes from one cell generation to the next. Therefore, it is imperative for cancer prevention that the cell division process is strictly regulated. In fission yeast and human cells, an actomyosin based contractile ring assembles at the medial region of the cell during mitosis and spatially dictates where the cell will physically separate. However, the molecular mechanisms that contribute to proper ring formation and function are unclear. The long- term goal of this project is to elucidate the regulatory controls necessary for a fully functional ring. Our preliminary results indicate that in fission yeast the Cdc14-like phosphatase, Clplp, interacts with Midlp, an anillin homolog required for correct ring placement. Utilizing biochemical assays and molecular imaging tech- niques, the objective of this application is to characterize these two ring components and their interaction in order to understand the contractile ring at a molecular level. It is expected that examining conserved proteins and molecular mechanisms that contribute to faithful cell division in simple systems will expedite the generation of knowledge necessary to significantly improve our understanding of cell division in higher organisms.

描述（由申请人提供）：物理细胞的平均分裂直接影响一套完整的染色体从一个细胞代转移到下一个细胞代。因此，对于癌症预防来说，严格调节细胞分裂过程至关重要。在分裂酵母和人类细胞中，基于肌动球蛋白的收缩环在有丝分裂期间在细胞的中间区域组装，并在空间上指示细胞将物理分离的位置。然而，分子机制，有助于适当的环的形成和功能尚不清楚。该项目的长期目标是阐明一个功能齐全的戒指所需的监管控制。我们的初步结果表明，在分裂酵母的Cdc14样磷酸酶，Clplp，与Midlp，正确的环位置所需的苯胺同系物相互作用。利用生物化学测定和分子成像技术，本申请的目的是表征这两种环组分及其相互作用，以便在分子水平上理解收缩环。预计研究有助于在简单系统中忠实细胞分裂的保守蛋白质和分子机制将加快产生必要的知识，以显着提高我们对高等生物细胞分裂的理解。

项目成果

NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement.

NDR 激酶 Sid2 从膜上驱动 Anillin 样 Mid1，以促进细胞分裂和内侧分裂位点放置。

• DOI: 10.1016/j.cub.2019.01.075
• 发表时间: 2019
• 期刊: Current biology : CB
• 作者: Willet,AlainaH;DeWitt,AshleyK;Beckley,JanelR;Clifford,DawnM;Gould,KathleenL
• 通讯作者: Gould,KathleenL