Leica TCS SP2 AOBS UV Confocal System

Leica TCS SP2 AOBS 紫外共焦系统

• 批准号: 6876961
• 负责人: Ivan Julian Dmochowski
• 金额: $ 49.73万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876961
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

DESCRIPTION (provided by applicant): Advances in biomedical research funded by the NIH in our School of Arts and Sciences (SAS) and Engineering School require ready access to a state-of-the-art confocal laser scanning microscope. Numerous currently supported projects within Chemistry, Physics, Biology, and Bioengineering at the University of Pennsylvania would benefit from this instrument. These include in vivo studies of polymersomes and related polymeric "worms" (Bioengineering); incorporating multiple porphyrin-based fluorophores into such vesicles, and localizing molecular beacons within cells (Chemistry); fate mapping zebrafish development through ultraviolet uncaging of fluorophores and small molecules (Biology); and investigating soft materials, neuronal interactions, and photodynamic therapy (Physics). The assembled user group possesses considerable expertise in optical imaging techniques, and, in particular, confocal laser scanning microscopy (CLSM). Routine access to CLSM is crucial for validating the localization of polymeric assemblies, proteins, and drugs within living cells and animals. CLSM provides hundreds-of-nanometer resolution images of complex 3-dimensional structures, and allows the interrogation of molecules with light, either within living systems or soft materials. Recent advances in commercially available confocal microscopes place these instruments increasingly in the domain of Chemistry and Biophysics. The current lack of a confocal microscope within the Chemistry Department, and the shortage of general-access, state-of- the-art CLSM facilities available to physical scientists at Penn negatively impacts studies important to many NIH-supported programs. An urgent need for access to these new technologies and a few shared scientific goals have brought together investigators from four different departments for this proposal.

描述（由申请人提供）：由美国国立卫生研究院资助的生物医学研究进展在我们的艺术与科学学院（SAS）和工程学院需要准备好使用最先进的共聚焦激光扫描显微镜。宾夕法尼亚大学的化学、物理、生物和生物工程领域目前支持的许多项目将受益于该仪器。这些包括聚合体和相关聚合“蠕虫”的体内研究（生物工程）；将多种卟啉基荧光团整合到这些囊泡中，并在细胞内定位分子信标（化学）；通过紫外线释放荧光团和小分子来绘制斑马鱼发育的命运图（生物学）；研究软材料、神经元相互作用和光动力疗法（物理学）。集合的用户组在光学成像技术方面拥有相当多的专业知识，特别是共聚焦激光扫描显微镜（CLSM）。常规获取CLSM对于验证活细胞和动物内聚合物组装、蛋白质和药物的定位至关重要。CLSM提供了数百纳米分辨率的复杂三维结构图像，并允许在生命系统或软材料中对分子进行光探测。最近在商用共聚焦显微镜方面取得的进展使这些仪器越来越多地应用于化学和生物物理学领域。目前化学系缺乏共聚焦显微镜，宾夕法尼亚大学物理科学家缺乏通用的、最先进的CLSM设备，这对许多nih支持的项目的重要研究产生了负面影响。为了获得这些新技术的迫切需要和一些共同的科学目标，来自四个不同部门的研究人员聚集在一起进行这项提案。

项目成果

Caged oligonucleotides for bidirectional photomodulation of let-7 miRNA in zebrafish embryos.

• DOI: 10.1016/j.bmc.2013.04.082
• 发表时间: 2013-10-15
• 期刊: BIOORGANIC & MEDICINAL CHEMISTRY
• 影响因子: 3.5
• 作者: Griepenburg, Julianne C.;Ruble, Brittani K.;Dmochowski, Ivan J.
• 通讯作者: Dmochowski, Ivan J.

Caging metal ions with visible light-responsive nanopolymersomes.

• DOI: 10.1021/la5036689
• 发表时间: 2015-01-20
• 期刊: Langmuir : the ACS journal of surfaces and colloids
• 作者: Griepenburg JC;Sood N;Vargo KB;Williams D;Rawson J;Therien MJ;Hammer DA;Dmochowski IJ
• 通讯作者: Dmochowski IJ

Ruthenium-Caged Antisense Morpholinos for Regulating Gene Expression in Zebrafish Embryos.

• DOI: 10.1039/c4sc03990d
• 发表时间: 2015-04-01
• 期刊: Chemical science
• 影响因子: 8.4
• 作者: Griepenburg JC;Rapp TL;Carroll PJ;Eberwine J;Dmochowski IJ
• 通讯作者: Dmochowski IJ

Transcriptome in vivo analysis (TIVA) of spatially defined single cells in live tissue.

• DOI: 10.1038/nmeth.2804
• 发表时间: 2014-02
• 期刊: NATURE METHODS
• 影响因子: 48
• 作者: Lovatt, Ditte;Ruble, Brittani K.;Lee, Jaehee;Dueck, Hannah;Kim, Tae Kyung;Fisher, Stephen;Francis, Chantal;Spaethling, Jennifer M.;Wolf, John A.;Grady, M. Sean;Ulyanova, Alexandra V.;Yeldell, Sean B.;Griepenburg, Julianne C.;Buckley, Peter T.;Kim, Junhyong;Sul, Jai-Yoon;Dmochowski, Ivan J.;Eberwine, James
• 通讯作者: Eberwine, James