Designer Molecular Probes for Biomedical Applications

用于生物医学应用的设计分子探针

• 批准号: 10552841
• 负责人: Ivan Julian Dmochowski
• 金额: $ 25万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10552841
• 关键词: Anesthesiology; Anesthetics; Area; Award; Awareness; Bioinorganic Chemistry; Biological; Biology; Biomedical Research; Book Chapters; Books; Brain; Cell physiology; Cells; Chemicals; Chemistry; Collaborations; Communities; Contrast Media; Development; Diabetes Mellitus; Diagnosis; Disease; Doctor of Philosophy; Education and Outreach; Fluorescent Probes; Funding; General Anesthesia; General anesthetic drugs; Genomics; Goals; Image; Immersion; Interdisciplinary Study; Laboratories; Legal patent; Light; Magnetic Resonance Imaging; Malignant Neoplasms; Mentors; Messenger RNA; Molecular; Molecular Probes; NMR Spectroscopy; Nerve Degeneration; Oligonucleotide Probes; Oligonucleotides; Organ; Organic Chemistry; Peer Review; Pennsylvania; Play; Postdoctoral Fellow; Process; Productivity; Proteins; Publications; RNA; Radiology Specialty; Regulation; Research; Role; Science; Scientist; Services; Specificity; Study Section; Technology; Tissues; Training; Universities; Xenon; base; biophysical chemistry; biophysical techniques; career; chemical synthesis; community building; complex biological systems; design; disease diagnosis; engineering design; experience; faculty mentor; graduate student; human disease; improved; in vivo; innovation; molecular imaging; nanoscale; novel; professor; programs; spatiotemporal; tool; transcriptome; transcriptomics; undergraduate student

Project Summary/Abstract: The PI is the Alan MacDiarmid Term Professor of Chemistry at the University of Pennsylvania (UPenn), where in 2003 he launched a multidisciplinary research program developing molecular probes that have led to breakthroughs in Chemical Biology, Radiology, Cellular Genomics/Transcriptomics, and Anesthesiology. In the course of this research, the PI has mentored 9 postdoctoral fellows, 35 graduate students, and 50 undergraduates, with many winning national awards and most graduates pursuing careers in science and biomedicine. The PI's research draws on formal training in supramolecular organic chemistry (Harvard-Mainz), bioinorganic and biophysical chemistry (Caltech, Ph.D.), and biology/biomolecular imaging (Caltech, postdoc). The PI's immersion for the past 19 years in the biomedical research enterprise at UPenn has burnished this experience. Through key collaborations, the PI has tackled significant biomedical research questions, using the tools of chemical synthesis, protein and oligonucleotide chemistry, and bioanalytical chemistry. The PI's 105 peer-reviewed publications, 7 patents, 4 edited books and contributed book chapters highlight a track record of productivity, innovation, impact, and dissemination in the development and application of chemical tools for biomedical research. The PI's service, including current membership on the SBCA study section, confirms a strong commitment to scientific review, education, outreach, community building, and junior faculty mentoring. This MIRA award extends the PI's highly successful GM-funded research in three program areas: 1) molecular imaging using xenon- based MRI contrast agents; 2) cellular transcriptome-in-vivo-analysis and mRNA regulation using light-activated oligonucleotide probes; and, 3) elucidation of general anesthetic-protein interactions with novel fluorescent probes, ultrasensitive 129Xe NMR spectroscopy, and other biophysical methods. Significant goals of the next five-year period are to refine these probe- based technologies with world-class research-collaborators at UPenn and establish high-value probes that can be disseminated broadly to the biomedical research community. Collectively, these tools will advance our molecular understanding of normal biological/biomedical processes (including general anesthesia), and provide avenues for improved diagnosis and treatment of many human diseases, including cancer, neurodegeneration, and diabetes.

项目摘要/摘要： PI 是宾夕法尼亚大学化学系 Alan MacDiarmid 学期教授 （宾夕法尼亚大学），2003 年，他启动了一项多学科研究计划，开发分子 导致化学生物学、放射学、细胞学突破的探针 基因组学/转录组学和麻醉学。在本研究过程中，PI 指导博士后9名，硕士研究生35名，本科生50名，其中多名 获得国家奖项，大多数毕业生从事科学和生物医学职业。这 PI 的研究借鉴了超分子有机化学（哈佛-美因茨）的正规培训， 生物无机和生物物理化学（加州理工学院博士）以及生物学/生物分子成像 （加州理工学院，博士后）。 PI过去19年专注于生物医学研究 宾夕法尼亚大学的企业已经丰富了这一经验。通过关键合作，PI 已 使用化学合成、蛋白质等工具解决了重大的生物医学研究问题 以及寡核苷酸化学和生物分析化学。 PI 的 105 项同行评审 出版物、7 项专利、4 本编辑书籍和贡献的书籍章节凸显了 开发和应用中的生产力、创新、影响和传播 用于生物医学研究的化学工具。 PI 的服务，包括当前的会员资格 SBCA 研究部分确认了对科学审查、教育、推广、 社区建设和初级教师指导。该 MIRA 奖项进一步扩展了 PI 的高度 通用汽车公司资助的三个项目领域的研究取得了成功：1）使用氙气的分子成像 基于 MRI 造影剂； 2) 细胞体内转录组分析和mRNA调控 使用光激活寡核苷酸探针；以及，3) 全身麻醉蛋白的阐明 与新型荧光探针、超灵敏 129Xe NMR 光谱等的相互作用 生物物理方法。下一个五年的重要目标是完善这些调查 与宾夕法尼亚大学世界一流的研究合作者合作开发基于技术的技术，并建立高价值 可以广泛传播到生物医学研究界的探针。总的来说， 这些工具将增进我们对正常生物/生物医学过程的分子理解 （包括全身麻醉），并为改善诊断和治疗提供途径 许多人类疾病，包括癌症、神经退行性疾病和糖尿病。