Time-Of-Flight Instrument with Variable Ionization Sources

描述(申请人提供)：可变电离源飞行时间仪器：我们在宾夕法尼亚大学化学系的质谱学设施中发现了两个非常关键的问题：(1)缺乏常规测量各种化合物的高分辨率数据的能力；(2)缺乏常规分析较大合成分子和生物分子的能力。安捷伦要求的飞行时间(TOF)仪器将彻底解决这些关键问题。该仪器由飞行时间质谱仪和多个大气压电离源组成。TOF分析仪设计用于在50-12,000质量/电荷(m/z)范围内同时提供卓越的质量精度(&lt；3ppm)、高质量分辨率(&gt；13,000)、大动态范围(3.5年)和快速光谱采集速率(20-40光谱/秒)。大气压电离源包括电喷雾电离(ESI)、大气压化学电离(APCI)和基质辅助激光解吸电离(MALDI)。之所以选择该仪器，是因为它独一无二地结合了(1)在很大的m/z范围内确定准确质量的能力，(2)由于可互换的电离源而分析非常广泛的化合物类别的能力，以及(3)仪器和软件的设计允许以常规的步进式方式测量样品。这三个特性使该仪器非常适合宾夕法尼亚大学化学系的多用户环境。建议的仪器将满足NIH支持的12名研究人员(主要用户群体)的所有关键和目前尚未满足的需求，这些研究人员涉及合成和天然分子的设计、合成和评估，如小分子、小和天然产品、非天然低聚物、天然和修饰多肽、肽仿制、天然和人工寡核苷酸以及小蛋白质。据估计，在五年内，该仪器将对宾夕法尼亚大学化学系约20名由NIH资助的研究人员的研究计划产生重大影响。

DESCRIPTION (provided by applicant): Time-Of-Flight Instrument with Variable Ionization Sources: We identified two very critical problems in the mass spectrometry facility of the Department of Chemistry at the University of Pennsylvania: (1) A lacking capability to routinely measure high-resolution data for a broad range of compounds and (2) a lacking capability to routinely analyze larger synthetic molecules and biomolecules. The requested Time-Of-Flight (TOF) instrument from Agilent will completely solve these critical problems. The proposed instrument consists of a TOF mass analyzer and multiple atmospheric pressure ionization sources. The TOF analyzer is designed to simultaneously provide excellent mass accuracy (< 3 ppm), high mass resolution (> 13,000), large dynamic range (3.5 decades), and fast spectral acquisition rates (20-40 spectra/sec) for the mass/charge (m/z) range of 50-12,000. The multiple atmospheric pressure ionization sources include electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and matrix- assisted laser desorption/ionization (MALDI). The instrument was selected because it uniquely combines (1) the ability to determine accurate masses over a large m/z range, (2) the capability to analyze a very broad range of compound classes due to interchangeable ionization sources, and (3) because the design of the instrument and the software allow the measurement of samples in a routine walk-up fashion. These three properties make this instrument ideal for the multi-user environment in the Chemistry Department at the University of Pennsylvania. The proposed instrument will meet all the critical and currently unfulfilled needs of 12 NIH-supported researchers (major user group) concerned with the design, synthesis, and evaluation of synthetic and natural molecules such as small molecules, small and natural products, unnatural oligomers, natural and modified peptides, peptidomimetics, natural and artificial oligonucleotides, and small proteins. It is estimated that over five years, the instrument would impact significantly the research programs of around 20 NIH-funded investigators in the Chemistry Department at the University of Pennsylvania.